| Budget Amount *help |
¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
Fiscal Year 2017: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2016: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2015: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
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| Outline of Final Research Achievements |
Bone marrow stromal cells (BMSCs) play an important role in bone marrow environment. Recent evidences suggest that extracellular vesicles (EVs) act as a mediator of cell-cell interaction in hematologic neoplasms. To clarify the possible association between the cargo of BMSC-EVs and disease severity, we performed miRNA profiling in BMSC-EVs derived from MDS patients.
BMSCs from 29 MDS patients were obtained by classical adhesion methods. We tentatively separated MDS patients into two groups according to the IPSS-R: low-risk and high-risk group. We found that a subset of EV-miRNA was differentially expressed between the two groups. Among them, we in particular focus on EV-miR-101: the expression level was significantly lower in high-risk MDS. Cell-cell communication via EV-miR-101 may play in part some role between BMSC and MDS cells. Our study shed light on the biological relevance of BMSC-EV and EV-miRNA in MDS bone marrow microenvironment.
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