Development of novel diagnostics for hematological malignancy on basis of the exosome analysis
| Project/Area Number |
15H04303
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Tumor diagnostics
|
|---|
| Research Institution | Tokyo Medical University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
大屋敷 純子 東京医科大学, 医学部, 教授 (20191950)
梅津 知宏 東京医科大学, 医学部, 講師 (40385547)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
Fiscal Year 2017: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2016: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2015: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
|
|---|
| Keywords | miRNA / 細胞外小胞 / 骨髄異形成症候群 / 骨髄間質細胞 / エクソソーム / 造血器腫瘍 |
|---|
| Outline of Final Research Achievements |
Bone marrow stromal cells (BMSCs) play an important role in bone marrow environment. Recent evidences suggest that extracellular vesicles (EVs) act as a mediator of cell-cell interaction in hematologic neoplasms. To clarify the possible association between the cargo of BMSC-EVs and disease severity, we performed miRNA profiling in BMSC-EVs derived from MDS patients.
BMSCs from 29 MDS patients were obtained by classical adhesion methods. We tentatively separated MDS patients into two groups according to the IPSS-R: low-risk and high-risk group. We found that a subset of EV-miRNA was differentially expressed between the two groups. Among them, we in particular focus on EV-miR-101: the expression level was significantly lower in high-risk MDS. Cell-cell communication via EV-miR-101 may play in part some role between BMSC and MDS cells. Our study shed light on the biological relevance of BMSC-EV and EV-miRNA in MDS bone marrow microenvironment.
|
Report
(4 results)
Research Products
(43 results)
-
-
[Journal Article] Hidden FLT3-D835Y clone in FLT3-ITD-positive acute myeloid leukemia that evolved into very late relapse with T-lymphoblastic leukemia2017
Author(s)
Katagiri S, Umezu T, Asano M, Akahane D, Azuma K, Makishima H, Yoshida K, Watatani Y, Chiba K, Miyano S, Ogawa S, Ohyashiki JH, Ohyashiki K
-
Journal Title
Leukemia and Lymphoma
Volume: 3
Issue: 6
Pages: 1-4
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Molecular signatures that predict response to azacitidine treatment for myelodysplastic syndromes2017
Author(s)
Nannya Y, Takeda J, Sato S, Shiozawa Y, Shiraishi Y, Kataoka K, Chiba K, Tanaka H, Chiba S, Asou N, Kiyoi H, Imai K, Hirase C, Dobashi N, Kiguchi T, Nakao S, Ohyashiki K, Miyazaki Y, Naoe T, Makishima H, Miyano S, Kenichi Yoshida K, Ogawa S*
Organizer
59th ASH annual meeting and exposition
Related Report
Int'l Joint Research
-
-
[Presentation] RUNX2 super enhancer promotes the development of blastic plasmacytoid dendritic cell neoplasm. 21th RUNX meeting2017
Author(s)
Sashida G, Kubota S, Tokunaga K, Oshima M, Umezu T, Kanai A, Tan KT, Yang H, Iwanaga E, Asou N, Maeda T, Iwama A, Ohyashiki K, Osato M
Organizer
21th RUNX meeting
Related Report
Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Exosomal miRNA signature of bone marrow mesenchymal stromal cells derived from MDS patients.2015
Author(s)
Saitoh Y, Imanishi S, Umezu T, Yoshizawa S, Asano M, Fujimoto H, Akahane D, Yamamoto Y, Kobayashi C, Ohyashiki JH, Ohyashiki K
Organizer
第77回日本血液学会学術集会
Place of Presentation
金沢
Year and Date
2015-10-16
Related Report
-
-
-
-
-
-
-
-
-
-
