| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2017: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2016: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2015: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
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| Outline of Final Research Achievements |
In this study, we have attempted to develop a series of methods by which single cell multi-omics analysis is enabled. We have successfully developed a suite of single cell analytical methods for transcriptional start sites analysis and open chromatin analysis, even though single cell DNA methylation analysis was not enabled mainly due to the difficulties in strict control of the bisulfite reaction. We have applied the developed method to monitor the diverse expression regulations between individual cells. We described fluctuating transcriptional responses invoked by the anti-cancer drugs either in susceptible or resistant lung cancer cell lines. We further examined transcriptome data from clinical specimen using a public cancer genome database and found that similar response seems to also occur in vivo. In addition to applying the developed method for our own purposes, we provided it to a number of researchers via collaborations.
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