Establishment of next-generation CRISPR system for human functional genomics
| Project/Area Number |
15H04319
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genome biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Ui-Tei Kumiko 東京大学, 大学院理学系研究科(理学部), 准教授 (50213327)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000)
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| Keywords | CRISPR / dCas9 / base-pairing / thermodynamics / melting temperature / バイオテクノロジー / 遺伝子 / 核酸 / ゲノム / RNA |
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| Outline of Final Research Achievements |
This study aims to develop genome editing technology of next generation for human functional genomics by improving CRISPR method. The CRISPR system is a new genome editing technology based on a prokaryotic acquired immune system, and has attracted attention as a breakthrough technology applicable to all kinds of organisms. In the CRISPR system, a guide RNA that distinguishes a target genomic site with a complementary base sequence forms a complex with a Cas9 protein, and Cas9 cleaves a genomic region where the guide RNA forms base-pairing. Therefore, in this study, using dCas9 which has no genome cleavage activity, we succeeded in establishing a practical CRISPR technique with high genome editing efficiency by combining experimental method and bioinformatics method.
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Report
(4 results)
Research Products
(75 results)
