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Development of high-efficiency gene targeting technologies

Research Project

Project/Area Number 15H04323
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field System genome science
Research InstitutionYokohama City University

Principal Investigator

Noritaka Adachi  横浜市立大学, 生命ナノシステム科学研究科(八景キャンパス), 教授 (30264675)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2017: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2015: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Keywordsジーンターゲティング / ヒトゲノム / ゲノム編集 / DNA修復 / ゲノム修復
Outline of Final Research Achievements

In this research project, we have successfully identified a human protein that is essential for alternative end-joining, a minor pathway of DNA double-strand break repair. This discovery has led us to develop a unique system to suppress random integration of transfected DNA, making it possible to perform human cell gene targeting with 100% efficiency.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (9 results)

All 2017 2016 2015 Other

All Int'l Joint Research (1 results) Journal Article (8 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 8 results,  Open Access: 7 results,  Acknowledgement Compliant: 3 results)

  • [Int'l Joint Research] 南カリフォルニア大学/MDアンダーソンがん研究所(米国)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Dual loss of human POLQ and LIG4 abolishes random integration.2017

    • Author(s)
      Saito S, Maeda R, Adachi N.
    • Journal Title

      Nature Commun.

      Volume: 8 Issue: 1

    • DOI

      10.1038/ncomms16112

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mechanistic basis for increased human gene targeting by promoterless vectors: roles of homology arms and Rad54 paralogs.2017

    • Author(s)
      Saito S, Kurosawa A, Adachi N.
    • Journal Title

      FEBS J.

      Volume: 284 Issue: 17 Pages: 2748-2763

    • DOI

      10.1111/febs.14137

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Non-homologous DNA end joining and alternative pathways to double-strand break repair.2017

    • Author(s)
      Chang HHY, Pannunzio NR, Adachi N, Lieber MR.
    • Journal Title

      Nature Rev. Mol. Cell Biol.

      Volume: 18 Issue: 8 Pages: 495-506

    • DOI

      10.1038/nrm.2017.48

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Dual loss of human POLQ and LIG4 abolishes random integration2017

    • Author(s)
      Saito S, Maeda R, Adachi N.
    • Journal Title

      Nat. Commun.

      Volume: in press

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] TBP-like Protein (TLP) Disrupts the p53-MDM2 Interaction and Induces Long-lasting p53 Activation.2017

    • Author(s)
      Maeda R., Tamashira H., Takano K., Takahashi H., Suzuki H., Saito S., Hojima W., Adachi N., U ra K ., E ndo T . and *Tam ura T.
    • Journal Title

      J. Biol. Chem.

      Volume: 292 Issue: 8 Pages: 3201-3212

    • DOI

      10.1074/jbc.m116.763318

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Mutations in XRCC4 cause primordial dwarfism without causing immunodeficiency.2016

    • Author(s)
      Saito S, Kurosawa A, Adachi N.
    • Journal Title

      J Hum Genet.

      Volume: 印刷中 Issue: 8 Pages: 679-685

    • DOI

      10.1038/jhg.2016.46

    • NAID

      40020923702

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Advances in the Development of Gene-Targeting Vectors to Increase the Efficiency of Genetic Modification2016

    • Author(s)
      Saito S, Adachi N.
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 39 Issue: 1 Pages: 25-32

    • DOI

      10.1248/bpb.b15-00701

    • NAID

      130005116505

    • ISSN
      0918-6158, 1347-5215
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Construction and applications of exon-trapping gene-targeting vectors with a novel strategy for negative selection.2015

    • Author(s)
      Saito S, Ura K, Kodama M, Adachi N.
    • Journal Title

      BMC Res Notes.

      Volume: 8 Issue: 1 Pages: 278-278

    • DOI

      10.1186/s13104-015-1241-6

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant

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Published: 2015-04-16   Modified: 2022-09-26  

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