| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2019: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2018: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
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| Outline of Final Research Achievements |
The RNA virus initiates its replication through translation of its genome RNA by the ribosomes in cells. Then, the RNA synthetase, one of the translated products, replicates the genome RNA. To elucidate the underlying mechanisms thereof, we tried to recapitulate these steps of the viral life cycle in vitro. For this aim, we established a reconstituted translation system composed of human factors. By combining this system and the cryo-electron microscopy (cryo-EM) analysis, we unraveled that hepatic type C virus (HCV) RNA captures a translating ribosome to effectively generate its own viral products. The data obtained herein may be useful for development of anti-HCV pharmaceuticals.
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