Molecular mechanism of force-sensing system and gene expression.
| Project/Area Number |
15H04327
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2017: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
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| Keywords | 転写 / 遺伝子発現 / 力刺激 / 細胞質／核移行 / MKL2 / メカノトランスダクション / 心臓 / 弁 / 力覚 / Hippo |
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| Outline of Final Research Achievements |
We have found that force-sensitive transcriptional co-activator MKL2 shuttles from cytoplasm into nucleus in very rapid response to shear stress, which completes within 10 minutes. We also found that this response is mediated by purinergic receptor activation and subsequent Ca signaling. To explore further the roles played by MKL2, we successfully made Mkl2 KO mice, which shows interesting phenotypes in circulatory system, such as heart and vasculatures. These phenotypes can be explained by a loss of response to mechanical stress in circulation. In addition, we found other unexpected interesting phenotypes in eyes and energy metabolism. We are preparing a manuscript, in which all data found in this project will be included.
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Report
(4 results)
Research Products
(9 results)
