Investigating phosphoregulation of meiotic recombination using superresolution microscopy
| Project/Area Number |
15H04328
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Kyoto University |
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Principal Investigator |
Carlton Peter 京都大学, 生命科学研究科, 准教授 (20571813)
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| Research Collaborator |
SATO CARLTON Aya
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
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| Keywords | 減数分裂 / 線虫 / 高解像度顕微鏡 / 染色体 / リン酸化による制御 / 染色体ダイナミックス / 超解像度顕微鏡 / ホスファターゼ / 組み換え |
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| Outline of Final Research Achievements |
In this grant period we investigated control of meiotic prophase chromosome dynamics by phosphorylation in C. elegans. Our starting point was the phosphatase PPH-4.1, which we had previously identified as a critical regulator of at least 4 critical chromosome dynamics events. We identified SYP-1, a member of the synaptonemal complex, as an important phosphoprotein in meiosis. SYP-1 phosphorylation is required for correct chromosome segregation, acting to specify the region in which chromosome cohesion will be lost in the first meiotic division. Additionally, our data shows that SYP-1 phosphorylation is required to recruit Polo-like kinase to the chromosome central region, as well as to promote the correct distribution of genetic crossovers on chromosomes.
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Report
(4 results)
Research Products
(3 results)
