| Budget Amount *help |
¥12,090,000 (Direct Cost: ¥9,300,000、Indirect Cost: ¥2,790,000)
Fiscal Year 2017: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
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| Outline of Final Research Achievements |
Using the locus-specific chromatin immunoprecipitation (ChIP) technology, we identified many genomic regions interacting with the β-globin gene 5'HS5 region constitutively or inducibly by induction of differentiation with sodium butyrate (NaB). Expression of a group of genes in the vicinity of the interacting sites was induced by NaB stimulation, suggesting that NaB-induced interactions between genomic regions is involved in regulation of the cooperative gene expression program. In addition, in an effort to elucidate molecular mechanisms of dynamic regulation of interferon-inducible genes, we developed enChIP systems using different dCas9 orthologues and tags. We further showed that the yields of the novel enChIP systems are sufficiently high for their practical use.
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