Identification and characterization of interactions between genomic regions using the locus-specific chromatin immunoprecipitation technology
| Project/Area Number |
15H04329
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Molecular biology
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
Fujii Hodaka 大阪大学, 微生物病研究所, 招へい教授 (30302665)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
藤田 敏次 大阪大学, 微生物病研究所, 招へい准教授 (10550030)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥12,090,000 (Direct Cost: ¥9,300,000、Indirect Cost: ¥2,790,000)
Fiscal Year 2017: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
|
|---|
| Keywords | クロマチン / エピジェネティクス / ゲノム生化学 / enChIP / iChIP / 核内三次元構造 / ゲノム領域間相互作用 / 次世代シークエンス法 |
|---|
| Outline of Final Research Achievements |
Using the locus-specific chromatin immunoprecipitation (ChIP) technology, we identified many genomic regions interacting with the β-globin gene 5'HS5 region constitutively or inducibly by induction of differentiation with sodium butyrate (NaB). Expression of a group of genes in the vicinity of the interacting sites was induced by NaB stimulation, suggesting that NaB-induced interactions between genomic regions is involved in regulation of the cooperative gene expression program. In addition, in an effort to elucidate molecular mechanisms of dynamic regulation of interferon-inducible genes, we developed enChIP systems using different dCas9 orthologues and tags. We further showed that the yields of the novel enChIP systems are sufficiently high for their practical use.
|
Report
(4 results)
Research Products
(40 results)
