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Regulatory mechanisms of replication origin activation.

Research Project

Project/Area Number 15H04330
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Molecular biology
Research InstitutionOsaka University

Principal Investigator

Masukata Hisao  大阪大学, 理学研究科, 教授 (00199689)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Keywordsテロメア / 複製開始 / 細胞周期 / 分裂酵母 / shelterin / Taz1 / Rif1 / PP1 / 複製開始点 / 染色体 / 核内局在 / 複製タイミング / DNA複製 / クロマチン / 細胞核 / Mcm10 / デグロン
Outline of Final Research Achievements

We found that the N-terminal 119 amino acid region of fission yeast Sld3 interacts each other and stabilizes Sld3 protein in the cells. Thus, it contributes to formation of bidirectional replication forks in replication initiation. On the other hand, the Taz1-dependent late replication origins that are visualized by lacO/LacI-GFP are localized at the nuclear periphery throughout the interphase, and associate with the telomeres specifically in the G1/S phase. The shelterin components play roles in tethering of the internal replication origins to the telomeres. The telomeric association of the replication origins causes suppression of early initiation by Rif1 and PP1 that are enriched at the telomeres.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (30 results)

All 2018 2017 2016 2015 2014 Other

All Journal Article (7 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 7 results,  Open Access: 7 results,  Acknowledgement Compliant: 5 results) Presentation (18 results) (of which Int'l Joint Research: 13 results,  Invited: 7 results) Remarks (5 results)

  • [Journal Article] Nucleosomes around a mismatched base pair are excluded via an Msh2-dependent reaction with the aid of SNF2-family ATPase Smarcad1.2018

    • Author(s)
      Terui,R.,Nagao,K.,Kawasoe, Y., Taki,K., Higashi,TL., Tanaka,S., Nakagawa,T., Obusae,C., Masukata,H., Takahashi,TS.
    • Journal Title

      Genes and Development

      Volume: -

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Regulation of mitotic recombination between DNA repeats in centromeres.2017

    • Author(s)
      Zafar,F., Okita,AK. Onaka,AT., Su,J., Katahira,Y., Nakayama,J. Takahashi,TS., Masukata, H., Nakagawa, T.
    • Journal Title

      Nucleic Acids Research

      Volume: 44(22) Issue: 19 Pages: 11222-11235

    • DOI

      10.1093/nar/gkx763

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] MutSα maintains the mismatch repair capability by inhibiting PCNA unloading2016

    • Author(s)
      Kawasoe, Y., Tsurimoto, T., Nakagawa, T., Masukata, H., Takahashi, TS.
    • Journal Title

      eLIFE

      Volume: 12;5

    • DOI

      10.7554/elife.15155

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Rad51 and Rad54 promote noncrossover recombination between centromere repeats on the same chromatid to prevent isochromosome formation.2016

    • Author(s)
      Onaka, AT., Toyofuku, N., Inoue, T., Okita, AK., Sagawa, M., Su, J., Shitanda, T., Matsuyama, R., Zafar, F., Takahashi, TS., Masukata, H., Nakagawa, T.
    • Journal Title

      Nucleic Acids Research

      Volume: 44 (22) Issue: 22 Pages: 10744-10757

    • DOI

      10.1093/nar/gkw874

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Shugoshin forms a specialized chromatin domain at subtelomeres that regulates transcription and replication timing.2016

    • Author(s)
      Tashiro S, Handa T, Matsuda A, Ban T, Takigawa T, Miyasato K, Ishii K, Kugou K, Ohta K, Hiraoka Y, Masukata H, Kanoh J.
    • Journal Title

      Nature Commun.

      Volume: 7 Issue: 1 Pages: 10393-10393

    • DOI

      10.1038/ncomms10393

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Histone H4 acetylation required for chromatin decompaction during DNA replication2015

    • Author(s)
      Ruan K, Yamamoto TG, Asakawa H, Chikashige Y, Kimura H, Masukata H, Haraguchi T, Hiraoka Y.
    • Journal Title

      Sci Rep

      Volume: 5 Issue: 1 Pages: 1-10

    • DOI

      10.1038/srep12720

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Meiotic nuclear movements in fission yeast are regulated by the transcription factor Mei4 downstream of a Cds1-dependent replication checkpoint pathway.2014

    • Author(s)
      Ruan K, Yamamoto TG, Asakawa H, Chikashige Y, Masukata H, Haraguchi T, Hiraoka Y.
    • Journal Title

      Genes Cells

      Volume: 20 Issue: 3 Pages: 160-172

    • DOI

      10.1111/gtc.12207

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Spatial and temporal control of chromosomal DNA replication by telomere sequence and telomere binding proteins in the fission yeast nucleus2017

    • Author(s)
      升方久夫
    • Organizer
      The 9th International Fission Yeast Meeting.
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Spatial and temporal control of chromosome DNA replication by telomere binding proteins.2017

    • Author(s)
      小川志帆、城戸沙友里、小川英知、淺川東彦、高橋達郎、中川拓郎、平岡泰、升方久夫
    • Organizer
      Eukaryotic DNA Replication and Genome Maintenance
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] テロメア結合タンパク質およびシェルタリン構成因子によるDNA複製の時空間的制御機構の解明2017

    • Author(s)
      小川志帆、半田哲也、中村優太、高橋達郎、中川拓郎、小川英知、淺川東彦、平岡泰、升方久夫
    • Organizer
      第40回日本分子生物学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 分裂酵母シェルタリンによる複製開始タイミング制御機構2017

    • Author(s)
      城戸沙友里、小川志帆、高橋達郎、中川拓郎、升方久夫
    • Organizer
      第40回日本分子生物学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 分裂酵母における複製開始複合体形成の核内局在による制御2016

    • Author(s)
      升方久夫
    • Organizer
      第38回日本分子生物学会年会ワークショップ
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Control of DNA replication timing and location by telomere-binding proteins in fission yeast2016

    • Author(s)
      升方久夫
    • Organizer
      10th 3R International Symposium
    • Place of Presentation
      松江
    • Year and Date
      2016-11-13
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Control of subnuclear localization and replication timing by telomere binding proteins in fission yeast2016

    • Author(s)
      升方久夫
    • Organizer
      FASEB Science Research Conferences
    • Place of Presentation
      Snowmass, Colorado, USA
    • Year and Date
      2016-07-31
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Control of replication timing and subnuclear localization by telomere binding proteins in fission yeast2016

    • Author(s)
      升方久夫
    • Organizer
      DNA Metabolism, Genomic Stability and Disease、Cold Spring Harbor Asia
    • Place of Presentation
      Suzhou、China
    • Year and Date
      2016-06-13
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] テロメア結合タンパク質によるDNA複製の時空間的制御機構2015

    • Author(s)
      小川志帆、中村優太、高橋達郎、中川拓郎、小川英知、淺川東彦、平岡泰、升方久夫
    • Organizer
      第38回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] DNA複製とミスマッチ修復の機能的相関2015

    • Author(s)
      高橋達郎、河添好孝、釣本敏樹、中川拓郎、升方久夫
    • Organizer
      第38回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Replication timing control by telomere-adjacent nuclear localization.2015

    • Author(s)
      小川志帆、中村優太、半田哲也、小川英知、淺川東彦、高橋達郎、中川拓郎、平岡泰、升方久夫
    • Organizer
      Eukaryotic DNA Replication and Genome Maintenance
    • Place of Presentation
      Cold Spring Harbor, NY USA
    • Year and Date
      2015-09-01
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The mechanism of the MutSα-dependent strand memory reaction in Xenopus egg extract.2015

    • Author(s)
      高橋達郎、河添好孝、釣本敏樹、中川拓郎、升方久夫
    • Organizer
      Eukaryotic DNA Replication and Genome Maintenance
    • Place of Presentation
      Cold Spring Harbor, NY USA
    • Year and Date
      2015-09-01
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Replication timing control by telomere-adjacent nuclear localization in fission yeast.2015

    • Author(s)
      升方久夫、中村優太、小川英知、淺川東彦、高橋達郎、中川拓郎、平岡泰、小川志帆
    • Organizer
      第8回International Fission Yeast Meeting
    • Place of Presentation
      神戸
    • Year and Date
      2015-06-21
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Shugoshin forms a specialized chromatin domain at subtelomeres, repressing transcription and replication.2015

    • Author(s)
      田代三喜、半田哲也、松田厚志、坂琢人、瀧川徹、宮里和実、石井浩二郎、久郷和人、太田邦広、平岡泰、升方久夫、加納純子
    • Organizer
      第8回International Fission Yeast Meeting
    • Place of Presentation
      神戸
    • Year and Date
      2015-06-21
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] H3K9 methylation in heterochromatin suppresses gross chromosomal rearrangement in centromere in mitotic cells.2015

    • Author(s)
      中川拓郎、沖田暁子、高橋達郎、升方久夫
    • Organizer
      第8回International Fission Yeast Meeting
    • Place of Presentation
      神戸
    • Year and Date
      2015-06-21
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Temporal and spatial regulation of replication in fission yeast.2015

    • Author(s)
      小川志帆、中村優太、高橋達郎、中川拓郎、小川英知、淺川東彦、平岡泰、升方久夫
    • Organizer
      第8回International Fission Yeast Meeting
    • Place of Presentation
      神戸
    • Year and Date
      2015-06-21
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Rad51-independent recombination between centromere repeats causes gross chromosomal rearrangement in fission yeast.2015

    • Author(s)
      大仲惇司、片平泰弘、沖田暁子、浅井麗伊、高橋達郎、升方久夫、中川拓郎
    • Organizer
      第8回International Fission Yeast Meeting
    • Place of Presentation
      神戸
    • Year and Date
      2015-06-21
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] CENP-T histone-fold protein suppresses translocation in fission yeast.2015

    • Author(s)
      蘇傑、高橋達郎、升方久夫、中川拓郎
    • Organizer
      第8回International Fission Yeast Meeting
    • Place of Presentation
      神戸
    • Year and Date
      2015-06-21
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Remarks] 大阪大学大学院理学研究科生物科学専攻升方研究室

    • URL

      http://www.bio.sci.osaka-u.ac.jp/dbs01/re-paper-temp.php?id=14

    • Related Report
      2017 Annual Research Report
  • [Remarks] 大阪大学大学院理学研究科生物科学専攻升方研究室

    • URL

      http://www.bio.sci.osaka-u.ac.jp/bio_web/lab_page/masukata/thesis/index.html

    • Related Report
      2017 Annual Research Report
  • [Remarks] 大阪大学大学院理学研究科生物科学専攻升方研究室

    • URL

      http://www.bio.sci.osaka-u.ac.jp/bio_web/lab_page/masukata/index.html

    • Related Report
      2016 Annual Research Report
  • [Remarks] 大阪大学大学院理学研究科生物科学専攻分子遺伝学研究室

    • URL

      http://www.bio.sci.osaka-u.ac.jp/dbs01/re-paper-temp.php?id=14

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
  • [Remarks] 大阪大学大学院理学研究科生物科学専攻分子遺伝学升方研究室ホームページ

    • URL

      http://www.bio.sci.osaka-u.ac.jp/bio_web/lab_page/masukata/

    • Related Report
      2015 Annual Research Report

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Published: 2015-04-16   Modified: 2019-03-29  

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