Cyanobacterial strategy for survival under diurnal cycles with the clock system

• Project/Area Number: 15H04332
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Waseda University
• Principal Investigator: Iwasaki Hideo 早稲田大学, 理工学術院, 教授 (00324393)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000); Fiscal Year 2017: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2016: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
• Keywords: 概日リズム / シアノバクテリア / KaiC / 明暗サイクル / 減衰振動 / kaiA / rpaA / 明暗応答 / UV耐性 / 概日時計 / 日周適応

Outline of Final Research Achievements

The unicellular cyanobacterium, Synechococcus provides an excellent model to study circadian systems. We have demonstrated (i) Hopf bifurcation underlying the in vitro reconstituted KaiC phosphorylation cycle at low temperature, ensuring damped oscillation resonated to external 24-h period cycles, (ii) a damped oscillation in transcriptional regulation even in the absence of KaiA, and (iii) circadian UV-resistance rhythms associated with carbon metabolism.

Research Products

• [Journal Article] Low temperature nullifies the circadian clock in cyanobacteria through Hopf bifurcation (2017)
  • Author(s): Murayama Yoriko、Kori Hiroshi、Oshima Chiaki、Kondo Takao、Iwasaki Hideo、Ito Hiroshi
  • Journal Title: PNAS Volume: 114 Issue: 22 Pages: 5641-5646
  • DOI: 10.1073/pnas.1620378114
  • NAID: 120006220445
  • Peer Reviewed / Open Access
• [Journal Article] 生物時計の分子メカニズム研究の展開： 転写翻訳フィードバックループ・モデルを 巡って (2017)
  • Author(s): 岩崎秀雄
  • Journal Title: 科学（岩波） Volume: 12月号 Pages: 1121-1129
• [Journal Article] 座談会:いま学会・研究コミュニティーのあり方を議論しよう (2017)
  • Author(s): 水島 昇，杉本亜砂子，吉森 保，岩崎秀雄
  • Journal Title: 実験医学 Volume: 600号突破記念号
• [Journal Article] Cyanobacterial circadian clock is nullified by low temperature through Hopf bifurcation (2017)
  • Author(s): Murayama Y, Kori H, Oshima C, Kondo T, Iwasaki H, Ito H
  • Journal Title: Proc Natl Acad Sci USA Volume: in press
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The initiation of nocturnal dormancy in Synechococcus as an active process. (2015)
  • Author(s): Takano, S., Tomita, J., Sonoike, K. and Iwasaki, H.
  • Journal Title: BMC Biol. Volume: 13 Issue: 1 Pages: 36-36
  • DOI: 10.1186/s12915-015-0144-2
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Mathematical study of pattern formation accompanied by heterocyst differentiation in multicellular cyanobacterium (2015)
  • Author(s): Ishihara J, Tachikawa M, H. Iwasaki, Mochizuki A
  • Journal Title: J. theor. Biol. Volume: 371 Pages: 9-23
  • DOI: 10.1016/j.jtbi.2015.01.034
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Damped circadian oscillation in the absence of KaiA (2017)
  • Author(s): Hideo Iwasaki
  • Organizer: International Symposium "Circadian Clock of Cyanobacteria during 1991-2017"
  • Place of Presentation: Nagoya University
  • Year and Date: 2017-03-12
• [Presentation] シアノバクテリアの時計細胞システムを巡って：北山陽子さんが遺したもの (2017)
  • Author(s): 岩崎秀雄
  • Organizer: 北山陽子メモリアルシンポジウム「光合成生物の概日時計機構」
  • Place of Presentation: 名古屋大学
  • Year and Date: 2017-03-12
• [Presentation] シアノバクテリアの概日時計の万年野党的探究 (2017)
  • Author(s): 岩崎秀雄
  • Organizer: 藍藻の分子生物学2017
  • Invited
• [Presentation] aPrayer : the Memorial Monument to the Souls of Artificial Cells/Lives (2017)
  • Author(s): Hideo Iwasaki
  • Organizer: La vie Loeuvre: Nouvelles & ecologies, bioart, biodesign
  • Int'l Joint Research / Invited
• [Presentation] Identification of genes for circadian cell division control in cyanobacteria (2017)
  • Author(s): 川崎洸司，中村嘉博，羽谷慶汰，岩崎秀雄
  • Organizer: 日本時間生物学会
• [Presentation] Mutational analysis on growth defects by kaiA-nullification under short-day conditions (2017)
  • Author(s): 河本尚大，馬場響子，藪谷俊介，細川徳宗，岩崎秀雄
  • Organizer: 日本時間生物学会
• [Presentation] Genes for glycogen metabolism affect circadian UV resistance control in cyanobacteria (2016)
  • Author(s): 川崎洸司、岩崎秀雄
  • Organizer: 日本時間生物学会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2016-11-12
• [Presentation] A possible mechanism for the damped circadian transcriptional oscillation without KaiA in Synechococcus (2016)
  • Author(s): 河本尚大、岩崎秀雄
  • Organizer: 日本時間生物学会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2016-11-12
• [Presentation] 単細胞生物シアノバクテリアのkaiA 機能欠損株における減衰振動につ いて (2016)
  • Author(s): 河本尚大、藪谷俊介、細川徳宗、岩崎秀雄
  • Organizer: 時間生物フォーラム東京2016
  • Place of Presentation: 早稲田大学
  • Year and Date: 2016-03-16
• [Presentation] シアノバクテリアの概日時計と紫外線耐性 (2016)
  • Author(s): 川崎洸司、岩崎秀雄
  • Organizer: 時間生物フォーラム東京2016
  • Place of Presentation: 早稲田大学
  • Year and Date: 2016-03-16
• [Presentation] Circadian control of UV resistance in cyanobacteria (2015)
  • Author(s): 川崎洸司、岩崎秀雄
  • Organizer: 第22回日本時間生物学会学術大会
  • Place of Presentation: 東京大学
  • Year and Date: 2015-11-21
• [Presentation] Synechococcus elongatus PCC 7942の概日UV耐性リズムの制御メカニズムの解析 (2015)
  • Author(s): 川崎洸司、岩崎秀雄
  • Organizer: 藍藻の分子生物学2015
  • Place of Presentation: 千葉かずさDNA研究所
  • Year and Date: 2015-11-06
• [Presentation] Synechococcus elongatus PCC 7942のkaiA 機能欠損株における減衰振動について (2015)
  • Author(s): 河本尚大、藪谷俊介、細川徳宗、岩崎秀雄
  • Organizer: 藍藻の分子生物学2015
  • Place of Presentation: 千葉かずさDNA研究所
  • Year and Date: 2015-11-06
• [Remarks] 岩崎秀雄研究室
  • URL: http://www.waseda.jp/sem-iwasakilab/index.html
• [Remarks] 電気情報生命工学科岩崎秀雄研究室
  • URL: http://www.waseda.jp/sem-iwasakilab