| Budget Amount *help |
¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2015: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
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| Outline of Final Research Achievements |
For synthesizing a protein from DNA base sequence, it is necessary to correctly bind the amino acid to the transfer RNA (aminoacylation reaction), and usually the enzyme called aminoacyl tRNA synthetase (aaRS) plays its role. However, in methanogenic archaebacteria, an enzyme complex called transsulfursome (SepRS: SepCysE: SepCysS) does.
In this study, the reaction mechanism of this system was clarified using X-ray crystal structure analysis method, X-ray small angle scattering method, electron microscope observation and other biochemical methods.
Transsulfursome is thought to be the ancestral aminoacylation system. Therefore, it is expected that this research will go a step further to elucidate the mystery related to the birth of the genetic code in the future.
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