| Budget Amount *help |
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2017: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2015: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
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| Outline of Final Research Achievements |
ERp44 is a PDI-family member protein that transports back ER-resident enzymes and immature secretory proteins from the Golgi to the ER and thereby conducts protein quality control in the early secretory pathway. In this reserach project, we succeeded in crystal structure analysis of ERp44 at pH7.5 and pH6.5. Based on the structural information, we performed a series of biochemical, biophysical and cell biological studies. Consequently, we revealed the molecular mechanism by which ERp44 regulates the substrate binding and release in a pH-dependent manner (Watanabe et al., PNAS 2017).
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