Development of solid-state NMR structural study of retinal-binding site in rhodopsin with a chromophore
| Project/Area Number |
15H04336
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Yokohama National University |
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Principal Investigator |
Kawamura Izuru 横浜国立大学, 大学院工学研究院, 准教授 (20452047)
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| Co-Investigator(Kenkyū-buntansha) |
五東 弘昭 横浜国立大学, 大学院工学研究院, 准教授 (80635235)
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| Co-Investigator(Renkei-kenkyūsha) |
SUDO Yuki 岡山大学, 医歯薬学総合研究科, 教授 (10452202)
NAITO Akira 横浜国立大学, 大学院工学研究院, 名誉教授 (80172245)
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| Research Collaborator |
SHIGETA Arisu
MAKINO Yoshiteru
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2017: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2016: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
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| Keywords | 固体NMR / レチナール / ロドプシン / 膜タンパク質 / 細胞膜 / 13C安定同位体標識 / 化学シフト / 13C化学シフト / 固体NMR分光法 / 7回膜貫通型タンパク質 |
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| Outline of Final Research Achievements |
In order to investigate the retinal-binding pocket of microbial rhodopsin, we performed a investigation of the transformation from retinal oxime to retinal, the creation of 13C isotope-labeled retinal from Halobacteria and solid-state NMR study of retinal-binding pocket. The effective transformation from oxime to retinal was achieved. Consequently, segment 13C isotope labeled retinal was successfully created. The structure of retinal binding pocket in sensory rhodopsin II and Krokinobacter rhodopsin 2 were revealed by solid-state NMR spectroscopy. In addition, the structure of photo-intermediates in sensory rhodopsin II were revealed by in-situ photo-irradiation solid-state NMR.
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Report
(4 results)
Research Products
(89 results)
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[Journal Article] Structure and orientation of antibiotic peptide alamethicin in phospholipid bilayers as revealed by chemical shift oscillation analysis of solid state nuclear magnetic resonance and molecular dynamics simulationfion2015
Author(s)
Takashi Nagao, Daisuke Mishima, Namsrai Javlhalantugs, Jun Wang, Daisuke Ishioka, Kiyonori Yokota, Kazushi, Norisada, Izuru Kawamua, Kazuyoshi, Ueda, Akira Naito
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Journal Title
Biochim. Biophys. Acta
Volume: 1848
Issue: 11
Pages: 2789-2798
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] Solid-state NMR structural study of Tyr residue in the retinal binding-pocket of microbial rhodopsins (Oral).2016
Author(s)
I. Kawamura, R. Nishikawa, H. Seki, A. Shigeta, Y. Makino, T. Tsukamoto, T. Okitsu, A. Wada, Y. Sudo, A. Naito.
Organizer
17th International Conference on Retinal Proteins (ICRP2016)
Place of Presentation
Neues Palais, Potsdam
Year and Date
2016-10-02
Related Report
Int'l Joint Research
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[Presentation] Structure of retinal-binding pocket of microbial rhodopsin as revealed by solid-state NMR2016
Author(s)
I. Kawamura, R. Nishikawa, H. Seki, A. Shigeta, Y. Otani, S. Nakatani, Y. Makino, T. Okitsu, A. Wada, Y. Sudo, A. Naito
Organizer
XXVIIth International Conference on Magnetic Resonance in Biological Systems (ICMRBS2016)
Place of Presentation
京都国際会議場
Year and Date
2016-08-21
Related Report
Int'l Joint Research
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