Structural biology of Shigella effectors to elucidate the molecular mechanisms of substrate recognition and infection
| Project/Area Number |
15H04341
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | University of Hyogo |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KIM Minsoo 京都大学, 白眉センター, 特定准教授 (50466835)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2017: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
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| Keywords | エフェクター / X線結晶構造解析 / ユビキチン修飾経路 / 赤痢菌 / ユビキチンリガーゼ / 蛋白質 / 細菌 / IpaH / Ｘ線結晶構造解析 |
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| Outline of Final Research Achievements |
Pathogenic bacteria such as Shigella, deliver a variety of virulence factors, called effectors, into host cells via the type III secretion system. These effectors mimic or hijack host proteins and modulate host signaling pathways to promote bacterial infection. To elucidate the molecular mechanisms of Shigella flexneri effectors such as OspI and IpaH9.8, we performed the structural and functional analyses of them. OspI deamidates of Ubc13, thereby disrupting TRAF6 activation and dampening host inflammatory responses. IpaH9.8 and IpaH1.4 possessing E3 ligase activity, dampen the NF-kappaB-mediated inflammatory response. We determined the crystal structures of deamidated Ubc13 and substrate recognition domain of IpaH9.8. These structures provide the substrate recognition and inhibition mechanisms of immune response.
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Report
(4 results)
Research Products
(21 results)
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[Presentation] Structural Analysis of Reaction Mechanism of Shigella flexneri effector OspI2016
Author(s)
Nishide, A., Kim, M., Takagi, K., Sasakawa, C., Mizushima, T.
Organizer
The Annual Evaluation Conference of the Leading Program, University of Hyogo for the School Year of 2015
Place of Presentation
先端科学技術支援センター (兵庫・赤穂郡上郡町)
Year and Date
2016-03-14
Related Report
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