Budget Amount *help |
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2017: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
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Outline of Final Research Achievements |
Intramembrane cleaving proteases (I-CLiPs) are known to act in a variety of important cellular processes in diverse organism. RseP, an Escherichia coli S2P family intramembrane cleaving protease, is involved in regulation of the extracytoplasmic stress response and membrane quality control through specific cleavage of substrates within the lipid bilayer. We have shown that, in addition to the periplasmic PDZ domain that acts as a size-exclusion filter, the cytoplasmic MRE β-loop region and the C1N region play critical roles in specific recognition and substrate cleavage.
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