Verification of unit raft hypothesis by high-resolution single-molecule imaging
| Project/Area Number |
15H04351
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Gifu University (2017)
Kyoto University (2015-2016) |
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Principal Investigator |
Suzuki Kenichi 岐阜大学, 研究推進・社会連携機構, 教授 (50423059)
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| Co-Investigator(Renkei-kenkyūsha) |
ANDO Hiromune 岐阜大学, 研究推進・社会連携機構, 教授 (20372518)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2017: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2015: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
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| Keywords | ラフト / １分子観察 / GPIアンカー型受容体 / 糖脂質 / シグナル伝達 / GPIアンカー型タンパク質 / 細胞膜ドメイン / 糖鎖 / 細胞・組織 / 生物物理 / 脂質 / 1分子計測 |
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| Outline of Final Research Achievements |
In this study, we examined the hypothesis that raft-associated glycolipids and GPI-anchored receptors (GARs) form homodimers via specific interactions, and work as a unit for downstream signal transduction.
We found that a variety of glycolipids and GARs formed homodimers via specific interactions, which can be generalized. We also found that the homodimers of glycolipids and GARs were stabilized by lipid interactions. Furthermore, our results suggest that these homodimers work as a unit for inducing downstream signaling events or for regulating activity of membrane receptors.
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Report
(4 results)
Research Products
(32 results)
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[Journal Article] Raft-based sphingomyelin interactions revealed by new fluorescent sphingomyelin analogs2017
Author(s)
Masanao Kinoshita, Kenichi GN Suzuki, Nobuaki Matsumori, Misa Takada, Hikaru Ano, Kenichi Morigaki, Mitsuhiro Abe, Asami Makino, Toshihide Kobayashi, Koichiro M Hirosawa, Takahiro K Fujiwara, Akihiro Kusumi, Michio Murata
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Journal Title
J Cell Biol
Volume: 未定
Issue: 4
Pages: 1183-1204
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Raft-based interactions of gangliosides with a GPI-anchored receptor2016
Author(s)
N. Komura, K. G. N. Suzuki, H. Ando, M. Konishi, M. Koikeda, A. Imamura, R. Chadda, T. K. Fujiwara, H. Tsuboi, R. Sheng, W. Cho, K. Furukawa, K. Furukawa, Y. Yamauchi, H. Ishida, A. Kusumi (Co-Corresponding Author), and M. Kiso
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Journal Title
Nat. Chem. Biol.
Volume: in press
Issue: 6
Pages: 402-410
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Confined diffusion of transmembrane proteins and lipids induced by the same actin meshwork lining the plasma membrane2016
Author(s)
T. K. Fujiwara, Iwasawa, K., Kalay, Z., Tsunoyama, T. A. Y. Watanabe, Y. M. Umemura, H. Murakoshi, K. G. N. Suzuki, Y. L., Nemoto, N. Morone, A. Kusumi
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Journal Title
Mol. Cell Biol.
Volume: 27
Issue: 7
Pages: 1101-1119
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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