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New Frontier of Photosynthesis Research by Using the Cryogenic Optical Microscope

Research Project

Project/Area Number 15H04356
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionTohoku University

Principal Investigator

Shibata Yutaka  東北大学, 理学研究科, 准教授 (20300832)

Co-Investigator(Kenkyū-buntansha) 野口 巧  名古屋大学, 理学研究科, 教授 (60241246)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
Keywords共焦点顕微鏡 / 極低温 / 単一分子分光 / ステート遷移 / 光合成 / 励起スペクトル / 顕微分光 / 偏光異方性
Outline of Final Research Achievements

By using the developed cryogenic microscope system, intracellular inhomogeneous distributions of photosynthetic proteins of Chlamydomonas, a model organism for the photosynthesis study, were observed. The analysis clarified the extent of co-localizations of photosystem proteins and light-harvesting antenna proteins. This results demonstrated that antenna proteins actually shuttles between the two photosystem proteins upon state transition, which is a regulation mechanism of excitation balance of the two photosystems.
A system enabling scanning of the excitation wavelength was developed. By using the system, the fluorescence excitation spectra of single photosynthesis proteins were obtained for the first time. Information about the excitation-energy flow within the protein was obtained.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (33 results)

All 2018 2017 2016 Other

All Journal Article (6 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 6 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (25 results) (of which Int'l Joint Research: 12 results,  Invited: 5 results) Book (1 results) Remarks (1 results)

  • [Journal Article] In vivo energy transfer from bacteriochlorophyll c, d, e, or f to bacteriochlorophyll a in the wild-type and mutant cells of the green sulfur bacterium Chlorobaculum limnaeum2018

    • Author(s)
      J. Harada, Y. Shibata, M. Teramura, T. Mizoguchi, Y. Kinoshita, K. Yamamoto, H. Tamiaki
    • Journal Title

      ChemPhotoChem

      Volume: 2 Issue: 3 Pages: 190-195

    • DOI

      10.1002/cptc.201700164

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Single bovine serum albumin molecule can hold plural blue-emissive gold nanoclusters: A quantitative study with two-photon excitation2018

    • Author(s)
      Hamza Al Kindi, Ahmed Mohamed, Shinji Kajimoto, Nurbosyn Zhanpeisov, Hideyuki Horino, Yutaka Shibata, Izabela I. Rzeznicka, Hiroshi Fukumura
    • Journal Title

      J. Photochem. Photobiol. A

      Volume: 357 Pages: 168-174

    • DOI

      10.1016/j.jphotochem.2018.02.029

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Fluorescence property of photosystem II protein complexes bound to a gold nanoparticle2017

    • Author(s)
      K. Tahara, A. Mohamed, K. Kawahara, R. Nagao, Y. Kato, H. Fukumura, Y. Shibata, T. Noguchi
    • Journal Title

      Faraday Discussions

      Volume: 印刷中 Pages: 121-134

    • DOI

      10.1039/c6fd00188b

    • NAID

      120006343676

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report 2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Highly Enhanced Emission of Visible Light from Core-Dual-Shell-Type Hybridized Nanoparticles2017

    • Author(s)
      Sakamoto Naonari、Onodera Tsunenobu、Dezawa Takuma、Shibata Yutaka、Oikawa Hidetoshi
    • Journal Title

      Particle & Particle Systems Characterization

      Volume: 34 Issue: 12 Pages: 1700258-1700258

    • DOI

      10.1002/ppsc.201700258

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Red shift in the spectrum of a chlorophyll species is essential for the drought-induced dissipation of excess light energy in a poikilohydric moss, Bryum argenteum2017

    • Author(s)
      Shibata Yutaka、Mohamed Ahmed、Taniyama Koichiro、Kanatani Kentaro、Kosugi Makiko、Fukumura Hiroshi
    • Journal Title

      Photosynthesis Research

      Volume: 136 Issue: 2 Pages: 229-243

    • DOI

      10.1007/s11120-017-0461-0

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structure based modeling of fluorescence kinetics of photosystem II: Relation between its dimeric form and photoregulation2016

    • Author(s)
      A. Mohamed, R. Nagao, T. Noguchi, H. Fukumura, Y. Shibata
    • Journal Title

      J. Phys. Chem. B

      Volume: 120 Issue: 3 Pages: 365-376

    • DOI

      10.1021/acs.jpcb.5b09103

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Peer Reviewed
  • [Presentation] 高分解極低温顕微分光法を用いてステート遷移におけるLHCII の膜内移動について検証する2018

    • Author(s)
      藤田祐輝,伊藤稚菜,柴田穣
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 極低温顕微分光法による光合成タンパク質アセンブリー中間体の探索2018

    • Author(s)
      柴田穣,小林誉宗,千葉智史,永澤廣知
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 光化学系I の極低温単一分子分光2018

    • Author(s)
      小林誉宗,Sankar Jana,Du Ting,長尾遼,野口巧,柴田穣
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] The Photosynthetic Competences of Chlorosomes Containing Bacteriochlorophyll c, d, e, or f in the Green Sulfur Bacterium Chlorobaculum limnaeum Mutants Cells2017

    • Author(s)
      Jiro Harada, Yutaka Shibata, Tadashi Mizoguchi, Misaki Ryono, Misato Teramura, Ken Yamamoto, Hitoshi Tamiaki
    • Organizer
      2017 International Conference on Artificial Photosynthesis
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of scanning microscope for simultaneous measurements of emission and excitation spectra2017

    • Author(s)
      Sankar Jana, Yutaka Shibata
    • Organizer
      19th IUPAB congress and 11th EBSA congress
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Photosynthetic Antenna Complexes as Regulation Valve of Excitation Energy2017

    • Author(s)
      Yutaka Shibata
    • Organizer
      Physical Chemistry Colloquium on "Ultrafast electronic and structural dynamics"
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Single-Molecule Spectroscopy Study on Photosystem I at Low Temperatures2017

    • Author(s)
      Yutaka Shibata
    • Organizer
      8th International Conference "Photosynthesis and Hydrogen Energy Research for Sustainability"
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Study on shuttling of light-harvesting complexes upon state transition by using cryogenic optical microscope2017

    • Author(s)
      Yuki Fujita, Yutaka Shibata
    • Organizer
      8th International Conference "Photosynthesis and Hydrogen Energy Research for Sustainability"
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Microspectroscopy at cryogenic temperature - single molecules to living cells2017

    • Author(s)
      Yutaka Shibata
    • Organizer
      Tohoku-Melbourne Research Workshop on Science: Recent Advances in Physical Chemistry/Chemical Physics
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Study on shuttling of light-harvesting complexes upon state transition by using cryogenic optical microscope2017

    • Author(s)
      Yuki Fujita, Yutaka Shibata
    • Organizer
      Tohoku-Melbourne Research Workshop on Science: Recent Advances in Physical Chemistry/Chemical Physics
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of Novel Scanning Microscope for Excitation Spectra Measurement2017

    • Author(s)
      Sankar Jana, Takanori Kobayashi, Ryo Nagao, Takumi Noguchi, Yutaka Shibata
    • Organizer
      2017年光化学討論会
    • Related Report
      2017 Annual Research Report
  • [Presentation] タンパク質中金ナノクラスターの生成過程および発光特性2017

    • Author(s)
      出澤 拓磨, Hamza Al-kindi, Izabela Rzeznicka, 福村 裕史, 柴田 穣
    • Organizer
      Advanced Light Measurements and Life Sciences Seeking Bio. Phys. Chem. about the Triple Point, 最先端光計測とライフサイエンスの近未来 -Bio. Phys. Chem.三重点の探索
    • Related Report
      2017 Annual Research Report
  • [Presentation] 光化学系Iタンパク質複合体の単一分子蛍光分光2017

    • Author(s)
      柴田 穣,小林 誉宗,Du Ting,Sankar Jana,長尾 遼,野口 巧
    • Organizer
      第11回分子科学討論会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 光環境変化に応じた光捕集タンパク質の膜内移動を極低温顕微分光法で検出する2017

    • Author(s)
      藤田 祐輝,柴田 穣
    • Organizer
      第11回分子科学討論会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 光化学系Iの低温単一分子分光: ブリンキングの起源2017

    • Author(s)
      Sankar Jana, 小林 誉宗, Du Ting, 長尾 遼, 野口 巧, 柴田 穣
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Understanding Light-Harvesting and Energy-Dissipation Mechanisms in Natural Photosynthesis Based on the Protein Structure at the Atomic Level2016

    • Author(s)
      Yutaka Shibata, Ahmed Mohamed, Hiroshi Fukumura
    • Organizer
      9th Asian Photochemistry Conference 2016
    • Place of Presentation
      Singapre
    • Year and Date
      2016-12-04
    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] タンパク質中に生成した金ナノクラスターの発光特性 (Emission property of Au Nanoclusters Formed in a Protein)2016

    • Author(s)
      出澤 拓磨, Hamza Al-kindi, Izabela Rzeznicka, 福村 裕史, 柴田 穣
    • Organizer
      第54回日本生物物理学会年会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2016-11-25
    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
  • [Presentation] 緑化途上トウモロコシ生葉の極低温顕微分光による光合成タンパク質前駆体の蛍光スペクトル同定 (Spectral identification of late precursors to photosynthetic proteins by cryogenic microscopy of greening etiolated Zea mays leaves)2016

    • Author(s)
      永澤 廣知, 千葉 智史, 柴田 穣
    • Organizer
      第54回日本生物物理学会年会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2016-11-25
    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
  • [Presentation] Development of Novel Scanning Microscope for Measurement of Emission and Excitation Spectra Simultaneously2016

    • Author(s)
      Sankar Jana, 柴田 穣
    • Organizer
      第54回日本生物物理学会年会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2016-11-25
    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
  • [Presentation] 液体窒素温度における光化学系Iの単一分子分光2016

    • Author(s)
      柴田 穣, Du Ting, 長尾 遼, 野口 巧, Jana Sankar
    • Organizer
      2016年光化学討論会
    • Place of Presentation
      東京大学駒場キャンパス
    • Year and Date
      2016-09-06
    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
  • [Presentation] Does State Transition in Chlamydomonas reinhardtii Require Shuttling of Light-Harvesting Complexes between the Two Photosystems? Indication from Cryogenic Microscopy Study2016

    • Author(s)
      Yutaka Shibata, Yuki Fujita, Wakana Itoh, Kento Washiyama
    • Organizer
      17th International Congress on Photosynthesis Research
    • Place of Presentation
      Maastricht
    • Year and Date
      2016-08-07
    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Single Molecule Spectroscopy of Photosystem I and Its Blinking Mechanism at Liquid N2 Temperature2016

    • Author(s)
      Sankar Jana, Ting Du, Ryo Nagao, Takumi Noguchi, Yutaka Shibata
    • Organizer
      17th International Congress on Photosynthesis Research
    • Place of Presentation
      Maastricht
    • Year and Date
      2016-08-07
    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Drought-induced fluorescence quencher in a poikilohydric moss, Breum argenteum, is formed in the photosystem II core complex2016

    • Author(s)
      Yutaka Shibata, Ahmed Mohamed, Koichiro Taniyama, Kentaro Kanatani, Makiko Kosugi, Hiroshi Fukumura
    • Organizer
      ight-Harvesting Satellite Meeting of the 17th International Congress on Photosynthesis Research
    • Place of Presentation
      Egmond aan Zee
    • Year and Date
      2016-08-04
    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 光合成光捕集過程を精密科学として理解することを目指して2016

    • Author(s)
      柴田 穣
    • Organizer
      第24回 光合成セミナー2016:反応中心と色素系の多様性
    • Place of Presentation
      龍谷大学
    • Year and Date
      2016-07-09
    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Invited
  • [Presentation] Single Molecule Spectroscopy of Photosynthetic Protein at Cryogenic Temperatures2016

    • Author(s)
      Yutaka Shibata
    • Organizer
      6th IUPAC Symposium on Photochemistry
    • Place of Presentation
      Osaka City Central Public Hall
    • Year and Date
      2016-04-04
    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Int'l Joint Research
  • [Book] テイツ/ザイガー 植物生理学・発生学 原著第6版(翻訳)2017

    • Author(s)
      リンカーン・テイツ、エドゥアルド・ザイガー、イアン.M・モーラー、アンガス・マーフィー、西谷 和彦、島崎 研一郎
    • Total Pages
      832
    • Publisher
      講談社
    • ISBN
      4061538969
    • Related Report
      2017 Annual Research Report
  • [Remarks]

    • URL

      http://web.tohoku.ac.jp/orgphys/index.html

    • Related Report
      2017 Annual Research Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

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