|Budget Amount *help
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2018: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2016: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
|Outline of Final Research Achievements
To investigate flexibility of multi-domain proteins by advance molecular simulation, we developed a method to efficiently simulate structure variations of multi-domain proteins, generate conformational transition pathways, and calculate free energy landscape. In the application of this method to FlhAc, a bacterial flagellar export apparatus protein, we found that a temperature sensitive mutant G368C does not show significant difference at 300 K but is stabilized to a closed form in which domain 2 and 4 are in contact. Since the open form of FlhAc involves with its function as the export apparatus, stabilization of the closed form in this mutant is expected to suppress its function.