| Budget Amount *help |
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2017: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
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| Outline of Final Research Achievements |
1. Fluorescently labeled miRNA precursors were microinjected into a cell. They were matured by endogenous biosynthetic pathway and they were incorporated into RISC. Single-molecule imaging technique revealed that miRNAs move on actin filaments.
2. Localization of mRNAs labeled with Cy5-linear antisense DNA probe was investigated under the stress conditions. Super-resolution microcopy revealed that mRNAs are not uniformly present in the stress granule (SG) but are accumulated to form high density regions with a diameter of about 100 nm.
3. The lifetimes of translational arrest of SecM mutants, whose amino acids outside ribosome were partially truncated, were investigated. Amino acid residues 57-73 and 57-98 contributed to the stabilization of translational arrest.
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