| Budget Amount *help |
¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2017: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2016: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2015: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
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| Outline of Final Research Achievements |
Proteins solubiｌity and aggregation have long been suspected to influence the immune response against small size proteins, but no direct experimental evidence have been reported so far. One reason for the lack of experimental support is that changing protein solubility requires changing the solution condition, which is not possible in vivo. In our study, we used short Solubility Controlling Peptide tags (SCP tag) peptide tags attached to the termini of our model proteins (Bovine Trypsin Pancreatic Trypsin Inhibitor, and Dengue Envelope protein domain 3) for modulating their solubility without affecting their function or biophysical properties. We showed that some SCP tags can oligomerize the proteins into 30-50 mers in a reversible manner, and that these aggregates increase their immunogenicity. In future, we plan to further investigate the molecular mechanisms of immune response increase induced by SCP tags, and explore its possible biotechnological applications.
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