| Budget Amount *help |
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2017: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2016: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2015: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
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| Outline of Final Research Achievements |
To elucidate the mechanisms of pore formation induced by antimicrobial peptides (AMPs) such as magainin 2 and entry of cell-penetrating peptides (CPPs) such as transportan 10 (TP10) into vesicle lumen, we investigated the effects of several factors such as membrane tension on these functions using the single giant unilamellar vesicle method. We found that a magainin 2-induced pore is a stretch-activated pore and constructed a quantitative model of the initial stage of magainin 2-induced pore formation. We found the effects of mechanical properties of lipid bilayer (such as tension and cholesterol) on entry of TP10 into single GUVs, and proposed a hypothesis on the mechanism of entry of TP10 across lipid bilayers. We also revealed the elementary processes of functions of other AMPs (lactoferricin B and lactoferricin B (4-9)) and CPPs (oligoarginine).
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