| Budget Amount *help |
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
|
|---|
| Outline of Final Research Achievements |
Enterococcus hirae V-ATPase (EhVoV1) is a rotary molecular motor which functions as a sodium ion pump across the biological membrane. The hydrophilic potion EhV1 converts chemical energy of ATP hydrolysis into unidirectional mechanical rotation. To understand chemo-mechanical coupling mechanism of Enterococcus hirae V1 (EhV1), we conducted single-molecule rotation assay using 40-nm gold nanoparticle as a low-load probe. We found that 120° steps per ATP hydrolysis were further divided into 40° and 80° substeps. Based on the results obtained in this study and insight of the previous structural analysis by X-ray crystallography, we proposed a complete chemo-mechanical coupling scheme of EhV1.
|
