Organ size regulation - An approach by C. elegans genetics

• Project/Area Number: 15H04379
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Kwansei Gakuin University
• Principal Investigator: Nishiwaki Kiyoji 関西学院大学, 理工学部, 教授 (30342827)
• Co-Investigator(Kenkyū-buntansha): 久保田 幸彦 東北大学, 生命科学研究科, 助教 (70333325)
• Co-Investigator(Renkei-kenkyūsha): SHIBATA Yukimasa 関西学院大学, 理工学部, 助教 (80314053)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000); Fiscal Year 2017: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2016: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2015: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
• Keywords: 器官サイズ制御 / 咽頭 / 線虫 / 細胞外マトリックス / 器官サイズ / 器官形成 / サイズ制御 / 発生・分化

Outline of Final Research Achievements

We analyzed the regulatory mechanism for organ size using C. elegans pharynx as a model system. The C. elegans pharynx consists of 62 cells involving muscle, marginal, neural, and gland cells. Using EMS mutagenesis, we isolated various mutants having an elongated pharynx phenotype. Genetic analyses of these mutants revealed that there are at least three independent mechanisms: (1) Regulation of apical extracellular matrix, (2) regulation of basal extracellular matrix, and (3) generation of extracellular reactive oxygen species. None of the mutants exhibited abnormalities in the pharyngeal cell number. Our findings indicate that extracellular molecular mechanisms act in a major role to regulate pharynx length.

Research Products

• [Journal Article] Organ Length Control by an ADAMTS Extracellular Protease in Caenorhabditis elegans (2016)
  • Author(s): Shibata, Y., Kawakado,Y., Hori,N., Tanaka, K., Inoue, R., Takano, T., Kubota, Y., Nishiwaki,K.
  • Journal Title: G3 Volume: 6 Issue: 5 Pages: 1449-1457
  • DOI: 10.1534/g3.116.028019
  • Peer Reviewed
• [Journal Article] Mg2+ Extrusion from Intestinal Epithelia by CNNM Proteins Is Essential for Gonadogenesis via AMPK-TORC1 Signaling in Caenorhabditis elegans. (2016)
  • Author(s): Ishii T, Funato Y, Hashizume O, Yamazaki D, Hirata Y, Nishiwaki K, Kono N, Arai H, Miki H.
  • Journal Title: PLoS Genet. Volume: 12 Issue: 8 Pages: e1006276-e1006276
  • DOI: 10.1371/journal.pgen.1006276
  • Peer Reviewed / Open Access
• [Journal Article] Control of the basement membrane and cell migration by ADAMTS proteinases: Lessons from C. elegans genetics. (2015)
  • Author(s): Kim, H.-S.and Nishiwaki, K.
  • Journal Title: Matrix Biology Volume: 44-46C Pages: 64-69
  • DOI: 10.1016/j.matbio.2015.01.001
  • Peer Reviewed / Open Access
• [Presentation] 線虫のMIG-17/ADAMTSプロテアーゼ欠損変異体の新規サプレッサー (2017)
  • Author(s): 中村 久子, 柴田 幸政, 西脇 清二
  • Organizer: 第４０回日本分子生物学会年会
• [Presentation] 線虫C. elegansにおけるROSを介した咽頭サイズ制御機構の解析 (2017)
  • Author(s): 足立 将大、坂東 里美、平本 和也、加嶋 紀彦、森部 弘樹、柴田 幸政、西脇 清二
  • Organizer: 第４０回日本分子生物学会年会
• [Presentation] 線虫の咽頭サイズを制御するgob-1と関連遺伝子の機能解析 (2017)
  • Author(s): 井上 領, 松井 那美香, 佐方 修一郎, 井関 晶太, 柴田 幸政, 西脇 清二
  • Organizer: 第４０回日本分子生物学会年会
• [Presentation] 線虫の生殖巣形態維持を制御するmig-36の解析 (2017)
  • Author(s): 横井 俊亮、岩田 明莉、山本 弥生、近藤 祥平、柴田 幸政、西脇 清二
  • Organizer: 第４０回日本分子生物学会年会
• [Presentation] 線虫C. elegansの咽頭にサイズ異常を示す新規変異体の単離と解析 (2017)
  • Author(s): 小柴優実、佐方修一郎、柴田幸政、西脇清二
  • Organizer: 第４０回日本分子生物学会年会
• [Presentation] Transdifferentiation is prevented by TLK-1 kinase that represses the level of histone variant H3.3 (2017)
  • Author(s): Yukimasa Shibata, Kiyoji Nishiwaki
  • Organizer: 21st International C. elegans Conference
  • Int'l Joint Research
• [Presentation] 線虫の咽頭サイズを制御するgob-1遺伝子の解析 (2016)
  • Author(s): 井上領、松井那美香、井関晶太、柴田幸政、西脇清二
  • Organizer: 第３９回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-11-30
• [Presentation] 線虫C. elegansにおけるROSを介した咽頭サイズ制御機構の解析 (2016)
  • Author(s): 足立将大、坂東里美、平本和也、加嶋紀彦、森部弘樹、柴田幸政、西脇清二
  • Organizer: 第３９回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-11-30
• [Presentation] 線虫のADAMTSプロテアーゼによる基底膜IV型コラーゲン制御の解析 (2016)
  • Author(s): 中村久子、高野智美、柴田幸政、西脇清二
  • Organizer: 第３９回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-11-30
• [Presentation] Regulation of pharynx size by apical and basal extracellular matrices in C. elegans. (2016)
  • Author(s): Shibata, Y., Sakata, S., Kawakado, Y., Hori, N., Tanaka, K., Inoue, R., Takano, T., Kubota, Y., Nishiwaki, K.
  • Organizer: CDB Symposium 2016
  • Place of Presentation: 理化学研究所（兵庫県神戸市）
  • Year and Date: 2016-03-28
  • Int'l Joint Research
• [Presentation] Cell-fate-determination mechanism that functions redundantly with BET family protein (2015)
  • Author(s): Shibata, Y., Konakawa, R., Sawa, H., Nishiwaki, K.
  • Organizer: 第３８回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場（兵庫県神戸市）
  • Year and Date: 2015-12-01
• [Presentation] Involvement of a ribosomal protein in the MIG-17/ADAMTS-dependent regulation of cell migration in C. elegans (2015)
  • Author(s): Kondo, S., Yamaoka, R., Kim, H.-S., Nishiwaki, K.
  • Organizer: 第３８回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場（兵庫県神戸市）
  • Year and Date: 2015-12-01
• [Presentation] pqn-74 regulates pharynx size in C. elegans (2015)
  • Author(s): Sakata, S., Tanaka, K., Iseki, M., Nishiwaki, K.
  • Organizer: 第３８回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場（兵庫県神戸市）
  • Year and Date: 2015-12-01
• [Presentation] The TFIIH and SWI/SNF complexes are required for the cell fate maintenance in C. elegans. (2015)
  • Author(s): Shibata, Y., Konakawa, R., Sawa, H., Nishiwaki, K.
  • Organizer: 20th International C. elegans Meeting
  • Place of Presentation: Los Angeles (USA)
  • Year and Date: 2015-06-24
  • Int'l Joint Research
• [Remarks] 西脇研究室ホームページ
  • URL: http://sci-tech.ksc.kwansei.ac.jp/~nishiwaki/
• [Remarks] 西脇研究室ＨＰ
  • URL: http://sci-tech.ksc.kwansei.ac.jp/~nishiwaki/