Cellular dynamics during collective cell migration
| Project/Area Number |
15H04380
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | National Institute for Basic Biology |
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Principal Investigator |
Ueno Naoto 基礎生物学研究所, 形態形成研究部門, 教授 (40221105)
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| Co-Investigator(Kenkyū-buntansha) |
鈴木 誠 基礎生物学研究所, 形態形成研究部門, 助教 (10533193)
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| Co-Investigator(Renkei-kenkyūsha) |
KOYAMA Hiroshi 基礎生物学研究所, 初期発生研究部門, 助教 (10530462)
SANO Masaki 東京大学, 大学院理学系研究科, 教授 (40150263)
TANAKA Motomu 京都大学, 物質―細胞統合システム拠点, 特定拠点教授 (00706814)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
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| Keywords | 胚葉形成 / 原腸形成 |
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| Outline of Final Research Achievements |
We aimed to understand the physical background of collective cell migration through the measurement of force filed using Xenopus mesoderm undergoing gastrulation. We first established the culture system in which mesoderm tissue migrate mono-directionally depending on the concentration gradient of a chemo-attractant SDF-1. Second, we visualized cell morphology and change in relative position of cells by labelling cell membrane and nucleus with GFP and measured force field by the traction force microscopy. Using these methodologies, we have been able to show that cells dynamically changes their positions in the collectively migrating tissue and that only a limited population of cells in the tissue generates force required for cell migration. Furthermore, by imaging intracellular Ca2+ dynamics which leads to the formation of cell protrusions such as lamellipodia, which in turn mediates force generation, has enabled to introduce those multiple parameters into mathematical models.
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] Distinct intracellular Ca2+ dynamics regulate apical constriction and differentially contribute to neural tube closure.2017
Author(s)
Suzuki, M., Sato, M., Koyama, H., Hara, Y., Hayashi, K., Yasue, N., Imamura, H., Fujimori, T., Nagai, T., Campbell, RE., Ueno, N.
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Journal Title
Development.
Volume: 144
Issue: 7
Pages: 1307-1316
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] SHISA6 Confers Resistance to Differentiation-Promoting Wnt/β-Catenin Signaling in Mouse Spermatogenic Stem Cells.2017
Author(s)
Tokue M, Ikami K, Mizuno S, Takagi C, Miyagi A, Takada R, Noda C, Kitadate Y, Hara K, Mizuguchi H, Sato T, Taketo MM, Sugiyama F, Ogawa T, Kobayashi S, Ueno N, Takahashi S, Takada S, Yoshida S.
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Journal Title
Stem Cell Reports.
Volume: 8
Issue: 3
Pages: 561-575
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Tracking dynamics of histone H3 lysine 9 acetylation in Xenopus laevis tail regeneration2016
Author(s)
Suzuki M, Takagi C, Miura S, Sakane Y, Sakuma T, Sakamoto N, Endo T, Kamei Y, Kimura H, Yamamoto T, Ueno N, Suzuki K
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Journal Title
Genes to Cells
Volume: Vol.21 (4)
Issue: 4
Pages: 358-369
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Differences in the Mechanical Properties of the Developing Cerebral Cortical Proliferative Zone between Mice and Ferrets at both the Tissue and Single-Cell Levels.2016
Author(s)
Nagasaka, A., Shinoda, T., Kawaue, T., Suzuki, M., Nagayama, K., Matsumoto, T., Ueno, N., Kawaguchi, A. and Miyata, T.
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Journal Title
Front Cell Dev. Biol.
Volume: 25
Pages: 139-139
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Conservation of structure and function in vertebrate c-FLIP proteins despite rapid evolutionary change.2015
Author(s)
Sakamaki, K., Iwabe, N., Iwata, H., Imai, K., Takagi, C., Chiba, K., Shukunami, C., Tomii, K., and Ueno, N.
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Journal Title
Biochemistry and Biophysics Report
Volume: 3
Pages: 175-189
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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