| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2015: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Outline of Final Research Achievements |
Despite its importance, Mg-dechelatase that catalyzes the first step of chlorophyll degradation pathway was not identified. In this study, we found that Mg-dechelatase is encoded by SGR gene and the dechelating activity was successfully measured with the recombinant protein expressed in E. coli. We also found that substrate specificity differs between SGR1 and SGRL. By these studies, all major enzymes of the chlorophyll metabolic pathway were finally identified. Furthermore, it was revealed that SGR can catalyze not only free chlorophyll but also chlorophyll bound to protein, thus playing a central role in the degradation of chlorophyll-binding proteins and photosystems.
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