Comprehensive study of chlorophyll metabolism based on enzyme identification
| Project/Area Number |
15H04381
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Hokkaido University |
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Principal Investigator |
TANAKA Ayumi 北海道大学, 低温科学研究所, 特任教授 (10197402)
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| Co-Investigator(Renkei-kenkyūsha) |
TAKABAYASHI ATSUSHI 北海道大学, 低温科学研究所, 助教 (90546417)
ITO HISASHI 北海道大学, 低温科学研究所, 助教 (50596608)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2015: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Keywords | 色素体機能 / 光合成 / クロロフィル / 代謝 |
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| Outline of Final Research Achievements |
Despite its importance, Mg-dechelatase that catalyzes the first step of chlorophyll degradation pathway was not identified. In this study, we found that Mg-dechelatase is encoded by SGR gene and the dechelating activity was successfully measured with the recombinant protein expressed in E. coli. We also found that substrate specificity differs between SGR1 and SGRL. By these studies, all major enzymes of the chlorophyll metabolic pathway were finally identified. Furthermore, it was revealed that SGR can catalyze not only free chlorophyll but also chlorophyll bound to protein, thus playing a central role in the degradation of chlorophyll-binding proteins and photosystems.
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Report
(4 results)
Research Products
(54 results)
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[Journal Article] The non-Mendelian green cotyledon gene in soybean encodes a small subunit of Photosystem II.2017
Author(s)
Kohzuma, K., Sato, Y., Ito, H., Okuzaki, A., Masuda, Y., Watanabe, M., Fukuoka, H., Yamatani, H., Kanazawa, A., Tabei, Y., Ikeuchi, M., Sakamoto, W., Tanaka, A., and Kusaba, M.
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Journal Title
Plant Physiology
Volume: 173
Issue: 4
Pages: 2138-2147
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] PSI-NPQ in higher plants2018
Author(s)
Makio Yokono, Atsushi Takabayashi, Junko Kishimoto, Tomomichi Fujita, Masakazu Iwai, Akio Murakami, Seiji Akimoto, Ayumi Tanaka
Organizer
第59回日本植物生理学会年会
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