Mechanisms of mRNA alternative splicing and ABA-responsive phosphosignaling
| Project/Area Number |
15H04383
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
Umezawa Taishi 東京農工大学, (連合)農学研究科(研究院), 准教授 (70342756)
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| Co-Investigator(Kenkyū-buntansha) |
平山 隆志 岡山大学, 資源植物科学研究所, 教授 (10228819)
坂田 洋一 東京農業大学, 生命科学部, 教授 (50277240)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2016: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2015: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
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| Keywords | アブシシン酸 / リン酸化 / スプライシング / プロテインキナーゼ / 乾燥耐性 / シロイヌナズナ / 植物ホルモン / シグナル伝達 / 発芽制御 / 植物 / 発芽 |
|---|
| Outline of Final Research Achievements |
Abscisic acid (ABA) is a major phytohormone which regulates stress responses in plants. It had been demonstrated that protein phosphorylation is important in ABA signal transduction pathway, and we found several mRNA splicing factors as phosphoproteins in response to ABA. To elucidate splicing events in response to ABA, we performed RNA-seq analysis using Arabidopsis WT and srk2dei mutant, and identified over 170 splice variants were regulated by ABA and SnRK2. We are going to functional analysis of splice variants in ABA signaling, and RNA-seq analysis using Arabidopsis seeds in the same way.
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Report
(4 results)
Research Products
(82 results)
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[Journal Article] Comprehensive quantification and genome survey reveal the presence of novel phytohormone action modes in red seaweeds2016
Author(s)
Mikami, K., Mori, I.C., Matsuura, T., Ikeda, Y., Kojima, M., Sakakibara, H., Hirayama, T.
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Journal Title
J. Applied Phycology
Volume: 未定
Issue: 4
Pages: 1-10
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Allantoin, a stress-related purine metabolite, can activate jasmonate signaling in a MYC2-regulated and abscisic acid-dependent manner2016
Author(s)
Takagi, H., Ishiga Y., Watanabe W., Konishi T., Egusa M., Akiyoshi N., Matsuura T., Mori I. C., Hirayama T., Kaminaka H., Shimada H. and Sakamoto A
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Journal Title
J. Ext. Bot.
Volume: 67
Issue: 8
Pages: 2519-2532
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Plant Raf-like kinase integrates abscisic acid and hyperosmotic stress signaling upstream of SNF1-related protein kinase2.2015
Author(s)
Saruhashi M, Kumar Ghosh T, Arai K, Ishizaki Y, Hagiwara K, Komatsu K, Shiwa Y, Izumikawa K, Yoshikawa H, Umezawa T, Sakata Y, Takezawa D.
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Journal Title
Proc. Natl. Acad. Sci. USA.
Volume: 112
Issue: 46
Pages: 6388-6396
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Community analysis of picocyanobacteria in an oligotrophic lake by cloning 16S rRNA gene and 16S rRNA gene amplicon sequencing2015
Author(s)
Fujimoto N, Mizuno K, Yokoyama T, Ohnishi A, Suzuki M, Watanabe S, Komatsu K, Sakata Y, Kishida N, Akiba M, Matsukura S.
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Journal Title
The Journal of General and Applied Microbiology
Volume: 61
Issue: 5
Pages: 171-176
DOI
NAID
ISSN
0022-1260, 1349-8037
Related Report
Peer Reviewed / Open Access
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[Presentation] Comparative phosphoproteomic analysis of abscisic acid response mutant of Physcomitrella patens2018
Author(s)
Yurie Hara, Shinnosuke Ishikawa,Anna Amagai, Mayuri Kuwamura, Fuminori Takahashi, Saho Mizukado, Naoyuki Sugiyama, Yasushi Ishihama, Daisuke Takezawa, Yoichi Sakata, Kazuo Shinozaki, Taishi Umezawa
Organizer
第59回日本植物生理学会年会
Related Report
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