Analysis of pollen tube guidance factors

• Project/Area Number: 15H04385
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Nagoya University
• Principal Investigator: Kanaoka Masahiro 名古屋大学, 理学研究科, 講師 (10467277)
• Co-Investigator(Kenkyū-buntansha): 佐藤 綾人 名古屋大学, トランスフォーマティブ生命分子研究所, 特任准教授 (10512428)
• Research Collaborator: Tsukamoto Ryoko
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000); Fiscal Year 2018: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2017: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2016: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
• Keywords: 花粉管 / 受精 / 有性生殖 / 多様性 / 花粉管ガイダンス / 生殖 / 化合物 / シグナル伝達 / ケミカルスクリーニング / 花粉管誘引 / 生殖隔離 / 植物有性生殖 / 受容体

Outline of Final Research Achievements

In this research period, I have analyzed the function of pollen tube attractant proteins and established in vitro pollen tube guidance assay system in novel plant species.

Academic Significance and Societal Importance of the Research Achievements

花粉管誘引に関わる因子を同定し、その性質を明らかにすることは、植物の受精を理解する上できわめて重要である。本研究において複数の誘引因子の性質が明らかになったことは、植物の受精を理解する上で重要であるのみならず、将来的には異種間交配といった育種場の応用も期待される。

Research Products

• [Int'l Joint Research] University of Zurich(スイス)
• [Int'l Joint Research] University of Zurich(スイス)
• [Int'l Joint Research] Fujian University(中国)
• [Journal Article] Comparison of mechanisms of reproductive interference in Taraxacum (2019)
  • Author(s): Takemori Akane、Naiki Akiyo、Takakura Ko-Ichi、Kanaoka Masahiro M、Nishida Sachiko
  • Journal Title: Annals of Botany Volume: 20 Issue: 6 Pages: 1-11
  • DOI: 10.1093/aob/mcz007
  • Peer Reviewed / Open Access
• [Journal Article] Fine-scale ecological and transcriptomic data reveal niche differentiation of an allopolyploid from diploid parents in Cardamine (2019)
  • Author(s): Akiyama Reiko、Sun Jianqiang、Hatakeyama Masaomi、Lischer Heidi E.L.、Briskine Roman V.、Hay Angela、Gan Xiangchao、Tsiantis Miltos、Kudoh Hiroshi、Kanaoka Masahiro M.、Sese Jun、Shimizu Kentaro K.、Shimizu-Inatsugi Rie
  • Journal Title: bioRxiv Volume: 600783 Pages: 600783-600783
  • DOI: 10.1101/600783
  • Int'l Joint Research
• [Journal Article] ANTH domain-containing proteins are required for the pollen tube plasma membrane integrity via recycling ANXUR kinases (2018)
  • Author(s): Muro Keita、Matsuura-Tokita Kumi、Tsukamoto Ryoko、Kanaoka Masahiro M.、Ebine Kazuo、Higashiyama Tetsuya、Nakano Akihiko、Ueda Takashi
  • Journal Title: Communications Biology Volume: 1 Issue: 1 Pages: 152-152
  • DOI: 10.1038/s42003-018-0158-8
  • Peer Reviewed / Open Access
• [Journal Article] Exocytosis-coordinated mechanisms for tip growth underlie pollen tube growth guidance (2017)
  • Author(s): Luo Nan、Yan An、Liu Gang、Guo Jingzhe、Rong Duoyan、Kanaoka Masahiro M.、Xiao Zhen、Xu Guanshui、Higashiyama Tetsuya、Cui Xinping、Yang Zhenbiao
  • Journal Title: Nature Communications Volume: 8 Issue: 1 Pages: 1-10
  • DOI: 10.1038/s41467-017-01452-0
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Cell?cell communications and molecular mechanisms in plant sexual reproduction (2017)
  • Author(s): Kanaoka Masahiro M.
  • Journal Title: Journal of Plant Research Volume: 131 Issue: 1 Pages: 37-47
  • DOI: 10.1007/s10265-017-0997-2
  • Peer Reviewed
• [Journal Article] Environmental sensing and morphological plasticity in plants (2017)
  • Author(s): Mizutani Miya, Kanaoka Masahiro M.
  • Journal Title: Seminars in Cell & Developmental Biology Volume: 1 Pages: 1-9
  • DOI: 10.1016/j.semcdb.2017.10.029
  • Peer Reviewed
• [Journal Article] Peptide signaling in pollen tube guidance (2015)
  • Author(s): MM Kanaoka, T Higashiyama
  • Journal Title: Current opinion in plant biology Volume: 28 Pages: 127-136
  • DOI: 10.1016/j.pbi.2015.10.006
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 花粉管誘引因子LUREsの機能解析とマイクロトムを用いた花粉管誘引実験系の開発 (2018)
  • Author(s): 久林加奈、塚本涼子、東山哲也、金岡雅浩
  • Organizer: 第15回日本ナス科コンソーシアム
  • Int'l Joint Research
• [Presentation] 植物の受精と種間障壁を担う花粉管誘引因子の機能と多様性 (2018)
  • Author(s): 金岡 雅浩
  • Organizer: 日本発生生物学会 秋季シンポジウム2018
  • Invited
• [Presentation] 種選択的な花粉管ガイダンスに関わる誘引物質LUREsの機能解析 (2017)
  • Author(s): 金岡 雅浩、神谷 直、塚本 涼子、東山 哲也
  • Organizer: 日本植物学会第81回大会
• [Presentation] 異質倍数体植物にみる環境に応答した表現型と遺伝子発現の可塑性 (2017)
  • Author(s): 金岡 雅浩、青木 優里、水谷 未耶、秋山 礼子、清水 健太郎、清水（稲継） 理恵
  • Organizer: 遺伝学会第89回大会
• [Presentation] 花粉管ガイダンスを制御する分泌因子 (2016)
  • Author(s): 金岡雅浩、東山哲也
  • Organizer: 第57回植物生理学会年会
  • Place of Presentation: 岩手大学
  • Year and Date: 2016-03-19
  • Invited
• [Presentation] Phenotypic plasticity in relation to water environment in allotetraploid Cardamine flexuosa (2015)
  • Author(s): 金岡雅浩
  • Organizer: 日本進化学会第17回大会
  • Place of Presentation: 中央大学
  • Year and Date: 2015-08-21
  • Invited
• [Remarks] 生殖分子情報学研究室
  • URL: http://www.higashiyama-lab.com/