Spatial structure and mechanisms of plant responses to light

• Project/Area Number: 15H04389
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kyoto University
• Principal Investigator: NAGATANI Akira 京都大学, 理学研究科, 教授 (40183082)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000); Fiscal Year 2017: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2016: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
• Keywords: フィトクロム / 遺伝子発現制御 / 長距離シグナル / ネットワーク

Outline of Final Research Achievements

We developed novel methods to monitor gene expression in micro-tissue pieces and to perform micro laser dissection for the spatial analysis of the shade avoidance response. By adopting those method for the analysis of the shade avoidance response, we found that the responses substantially differed between cotyledon and hypocotyl or between mesophyll and vasculature. Furthermore, we revealed that auxin and an unknown signal independent of auxin play pivotal roles for the spatial regulation of the shade avoidance response.

Academic Significance and Societal Importance of the Research Achievements

植物の光応答における組織特異性や組織/器官間シグナル伝達に関する知見は限られていたが、本研究を実施することにより、組織や器官ごとの応答が分子レベルでどの程度異なるかが初めて明らかになった。また、避陰応答の空間的制御において、オーキシンやオ新規シグナルが果たす役割が明らかとなり、植物の光応答の空間構造に関する理解が深まった。これらの知見は、光受容体の働きを人為的に調整し植物の性質を改善する上で欠かせない。

Research Products

• [Journal Article] Chloroplasts Modulate Elongation Responses to Canopy Shade by Retrograde Pathways Involving HY5 and Abscisic Acid (2019)
  • Author(s): Ortiz-Alcaide Miriam、Llamas Ernesto、Gomez-Cadenas Aurelio、Nagatani Akira、Martinez-Garcia Jaime F.、Rodriguez-Concepcion Manuel
  • Journal Title: The Plant Cell Volume: 31 Issue: 2 Pages: 384-398
  • DOI: 10.1105/tpc.18.00617
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Auxin Contributes to the Intraorgan Regulation of Gene Expression in Response to Shade. (2018)
  • Author(s): Kim S, Mochizuki N, Deguchi A, Nagano AJ, Suzuki T, Nagatani A.
  • Journal Title: Plant Physiology Volume: 177 Issue: 2 Pages: 847-862
  • DOI: 10.1104/pp.17.01259
  • Peer Reviewed
• [Journal Article] Spatial regulation of the gene expression response to shade in Arabidopsis seedlings. (2015)
  • Author(s): Nito, K., T. Kajiyama, J. Unten-Kobayashi, A. Fujii, N. Mochizuki, H. Kambara and A. Nagatani
  • Journal Title: Plant Cell Physiol Volume: 56(7) Issue: 7 Pages: 1306-1319
  • DOI: 10.1093/pcp/pcv057
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Position-specific gene expression analysis using a microgram dissection method combined with on-bead cDNA library construction. (2015)
  • Author(s): Kajiyama, T., A. Fujii, K. Arikawa, T. Habu, N. Mochizuki, A. Nagatani and H. Kambara
  • Journal Title: Plant Cell Physiol Volume: 56(7) Issue: 7 Pages: 1320-1328
  • DOI: 10.1093/pcp/pcv078
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 1)Development and application of a high resolution imaging mass spectroscope for the study of plant tissues (2015)
  • Author(s): K.Takahashi, T.Kozuka, A.Anegawa, A.Nagatani, T.Mimura
  • Journal Title: PLANT AND CELL PHYSIOLOGY Volume: 56 Issue: 7 Pages: 1329-1338
  • DOI: 10.1093/pcp/pcv083
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Editorial: Emerging technologies for the study of plant environmental sensing. (2015)
  • Author(s): Nagatani A, Mimura T
  • Journal Title: Plant Cell Physiol Volume: 56(7) Issue: 7 Pages: 1249-1251
  • DOI: 10.1093/pcp/pcv095
  • Open Access
• [Presentation] Structural basis for the far-red high irradiance response of phytochrome A (2018)
  • Author(s): Akira Nagatani et al.
  • Organizer: International Symposium on Plant Photobiology (ISPP) 2018 (Matsue, Japan)
  • Int'l Joint Research / Invited
• [Presentation] Involvement of auxin in intra-organ regulation of gene expression responses to the shade stimulus (2018)
  • Author(s): Sujung Kim et al.
  • Organizer: International Symposium on Plant Photobiology (ISPP) 2018 (Matsue, Japan)
  • Int'l Joint Research
• [Presentation] How does a plant utilize different photoreceptors for the shade avoidance response? (2017)
  • Author(s): Akira Nagatani
  • Organizer: Asia and Oceania Conference on Photobiology (AOCP) 2017 (Seoul, Korea)
  • Int'l Joint Research / Invited
• [Presentation] Non-tissue autonomous regulation of auxin-responsive genes in the shade avoidance response (2017)
  • Author(s): Sujung Kim et al.
  • Organizer: Taiwan-Japan Plant Biology 2017 (Taipei, Taiwan)
  • Int'l Joint Research
• [Presentation] Red-light induced local gene expression responses during hook opening (2017)
  • Author(s): Isato Ohnishi et al.
  • Organizer: Kyoto-Bristol-Heidelberg Plant Sciences Worshop, Bristol, UK
• [Presentation] シロイヌナズナの子葉における避陰応答遺伝子のトランスクリプトーム解析 (2017)
  • Author(s): Sujung Kim 他
  • Organizer: 第58回 日本植物生理学会年会（鹿児島）
• [Presentation] シロイヌナズナの芽生えにおける避陰応答と低温応答の相互作用 (2017)
  • Author(s): 櫻井裕子 他
  • Organizer: 第58回 日本植物生理学会年会（鹿児島）
• [Presentation] The first step to understanding light-responses in natura (2016)
  • Author(s): Akira Nagatani; Ryota Otsuki; Yuko Sakurai; Nobuyoshi Mochizuki; Tomomi Suzuki
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手県盛岡市
  • Year and Date: 2016-03-18
  • Invited
• [Presentation] Spatial control of the shade avoidance response (2016)
  • Author(s): Akira Nagatani
  • Organizer: ICAR 2016, Gyeongju, Korea
  • Int'l Joint Research / Invited
• [Presentation] シロイヌナズナの子葉における避陰応答遺伝子の空間的な発現解析 (2016)
  • Author(s): Sujung Kim et al.
  • Organizer: 第80回 日本植物学会大会（沖縄）
• [Presentation] Inter-organ and inter-tissue communications in the shade avoidance response (2015)
  • Author(s): A. Nagatani
  • Organizer: Asia and Oceania Conference on Photobiology
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2015-11-15
  • Int'l Joint Research / Invited
• [Presentation] Spatiotemporal regulation of the shade avoidance response (2015)
  • Author(s): A. Nagatani
  • Organizer: BHK Symposium
  • Place of Presentation: Bristol, UK
  • Year and Date: 2015-11-05
  • Int'l Joint Research / Invited
• [Presentation] Spatial control of the shade avoidance response (2015)
  • Author(s): A. Nagatani
  • Organizer: 11th International Congress of Plant Molecular Biology
  • Place of Presentation: Iguazu Falls, Brazil
  • Year and Date: 2015-10-25
  • Int'l Joint Research / Invited
• [Presentation] Spatiotemporal regulation of the hypocotyl elongation in response to the shade stimulus (2015)
  • Author(s): A. Nagatani
  • Organizer: International Symposium on Plant Photobiology 2015
  • Place of Presentation: Austin, USA
  • Year and Date: 2015-05-26
  • Int'l Joint Research / Invited
• [Remarks] 京都大学大学院理学研究科植物生理学研究室ホームページ
  • URL: http://physiol2.bot.kyoto-u.ac.jp/HP3/index.html
• [Remarks] 京都大学大学院理学研究科植物学教室ホームページ
  • URL: http://www.bot.kyoto-u.ac.jp/j/index.html
• [Remarks] 研究室ホームページ
  • URL: http://physiol2.bot.kyoto-u.ac.jp/HP3/
• [Remarks] 教室ホームページ
  • URL: http://www.bot.kyoto-u.ac.jp/j/index.html