Analysisis of a novel gibberellin signaling pathway through calcium
| Project/Area Number |
15H04392
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2018: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2017: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 植物ホルモン / 信号伝達 / 転写制御 / 植物 / 転写調節 / シグナル伝達 / 発現制御 / キナーゼ |
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| Outline of Final Research Achievements |
DELLA proteins play a central role in gibberellin (GA) signaling. GA triggers DELLA degradation via the ubiquitin-proteasome pathway, thereby promoting plant growth. An increase in cytosolic Ca2+ was observed previously after several hours of GA application. Recent studies also suggest the existence of a DELLA-independent GA response. However, the effect of DELLA on the GA-induced increase in Ca2+ remains unknown. This study reexamined the effects of GAs on Ca2+ using the Ca2+ sensor protein in Arabidopsis. Ca2+ increased within a few minutes of GA treatment, even in transgenic plants expressing a mutated degradation-resistant version of DELLA and in della pentuple mutant plants. In addition, it was also revealed that Ca2+ is not involved in DELLA degradation. These results suggest that the GA-induced increase in Ca2+ occurs via a DELLA-independent pathway, providing important information on the GA signaling network.
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| Academic Significance and Societal Importance of the Research Achievements |
ジベレリン（GA）は植物の成長に顕著な促進作用を示すホルモンである。GAがいかにして植物の成長を制御しているのかを解明するためにGAの細胞内信号伝達に関する研究を行った。GA信号伝達において中心的な役割を果たしていると考えられているのが、DELLAタンパク質である。GAにより細胞内Ca2+濃度の上昇が速やかに誘導され、この誘導にはDELLAは関与しない事を明らかにした。本研究で見いだされたDELLAを介さないGA特異的な Ca2+濃度の上昇は未知のGA信号伝達経路の存在を示唆している。
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Report
(5 results)
Research Products
(37 results)
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[Journal Article] DELLA-GAF1 complex is a main component in gibberellin feedback regulation of GA 20-oxidase 22017
Author(s)
Fukazawa, J., Mori, M., Watanabe, S., Miyamoto, C., Ito, T. and Takahashi, Y.
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Journal Title
Plant Physiology
Volume: 175
Issue: 3
Pages: 1395-1406
DOI
Related Report
Peer Reviewed
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[Journal Article] DELLA-GAF1 complex is a main component in gibberellin feedback regulation of GA20ox2 in Arabidopsis2017
Author(s)
Fukazawa, J., Mori, M., Watanabe, S., Miyamoto, C., Ito, T. and Takahashi, Y.
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Journal Title
Plant Physiol.
Volume: 印刷中
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Binding of GID1 to DELLAs Promotes Dissociation of GAF1 from DELLA in GA Dependent Manner2015
Author(s)
Fukazawa, J., Ito, T., Yoshida, M., Kamiya, Y., Yamaguchi, S., and Takahashi, Y.
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Journal Title
Plant Signaling and Behavior
Volume: 10
Issue: 10
Pages: e1052923-e1052923
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Book] 植物生理学概論2017
Author(s)
桜井英博、柴岡弘郎、高橋陽介、小関良宏、藤田知道
Total Pages
246
Publisher
培風館
ISBN
9784563078256
Related Report
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