Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2018: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2017: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Outline of Final Research Achievements |
DELLA proteins play a central role in gibberellin (GA) signaling. GA triggers DELLA degradation via the ubiquitin-proteasome pathway, thereby promoting plant growth. An increase in cytosolic Ca2+ was observed previously after several hours of GA application. Recent studies also suggest the existence of a DELLA-independent GA response. However, the effect of DELLA on the GA-induced increase in Ca2+ remains unknown. This study reexamined the effects of GAs on Ca2+ using the Ca2+ sensor protein in Arabidopsis. Ca2+ increased within a few minutes of GA treatment, even in transgenic plants expressing a mutated degradation-resistant version of DELLA and in della pentuple mutant plants. In addition, it was also revealed that Ca2+ is not involved in DELLA degradation. These results suggest that the GA-induced increase in Ca2+ occurs via a DELLA-independent pathway, providing important information on the GA signaling network.
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