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Presence of wax components of Charophytes revealed by Genome and suface lipid analyses and their importance in adaptation to land environment

Research Project

Project/Area Number 15H04393
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphology/Structure
Research InstitutionTokyo Institute of Technology

Principal Investigator

Ohta Hiroyuki  東京工業大学, 生命理工学院, 教授 (20233140)

Co-Investigator(Kenkyū-buntansha) 堀 孝一  東京工業大学, 生命理工学院, 助教 (70453967)
佐々木 結子 (関本結子)  東京工業大学, 生命理工学院, 研究員 (60422557)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2017: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2016: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2015: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Keywords細胞外皮 / 細胞壁 / ワックス / 植物陸上進出 / 車軸藻植物門 / クレブソルミディウム / 細胞・組織 / 植物 / 進化 / 脂質 / 表層脂質 / クチクラ / トリアシルグリセロール / 陸上環境への適応
Outline of Final Research Achievements

In this study, we proposed that charophyte algae, which are the closest algae to land plants, acquired a cuticle-like hydrophobic layer composed of triacylglycerol to adapt to a terrestrial environment by initial land colonization. To demonstrate the hypothesis, we analyzed surface lipids and transcriptome of Klebsormidium, which early diverged from charophyte algae ancestor. As a result, it is assumed that surface structure and its related gene regulation were evolved in a stepwise manner during land colonization while using triacylglycerol as hydrophobic barrier. These results will provide information to clarify relation between process of land colonization by plants and evolution of its surface lipid.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (26 results)

All 2018 2017 2016 2015 Other

All Int'l Joint Research (3 results) Journal Article (7 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 7 results,  Open Access: 4 results,  Acknowledgement Compliant: 2 results) Presentation (16 results) (of which Int'l Joint Research: 8 results,  Invited: 4 results)

  • [Int'l Joint Research] Aust Res Coun Cent Excel Plant Ener Biol(Australia)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Xiamen University(China)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Universite Paris Diderot(France)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Physcomitrella MADS-box genes regulate water supply and sperm movement for fertilization2018

    • Author(s)
      Koshimizu Shizuka、Kofuji Rumiko、Sasaki-Sekimoto Yuko、Kikkawa Masahide、Shimojima Mie、Ohta Hiroyuki、Shigenobu Shuji、Kabeya Yukiko、Hiwatashi Yuji、Tamada Yosuke、Murata Takashi、Hasebe Mitsuyasu
    • Journal Title

      Nature Plants

      Volume: 4 Issue: 1 Pages: 36-45

    • DOI

      10.1038/s41477-017-0082-9

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Recurrent acquisition of cytosine methyltransferases into eukaryotic retrotransposons.2018

    • Author(s)
      de Mendoza A, Bonnet A, Vargas-Landin DB, Ji N, Li H, Yang F, Li L, Hori K, Pflueger J, Buckberry S, Ohta H, Rosic N, Lesage P, Lin S, Lister R
    • Journal Title

      Nature communications

      Volume: 9 Issue: 1

    • DOI

      10.1038/s41467-018-03724-9

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Primitive Auxin Response without TIR1 and Aux/IAA in the Charophyte Alga Klebsormidium nitens2017

    • Author(s)
      Kinuka Ohtaka, Koichi Hori, Yuri Kanno, Mitsunori Seo, Hiroyuki Ohta
    • Journal Title

      Plant Physiology

      Volume: 174 Issue: 3 Pages: 1621-1632

    • DOI

      10.1104/pp.17.00274

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Primitive Extracellular Lipid Components on the Surface of the Charophytic Alga Klebsormidium flaccidum and Their Possible Biosynthetic Pathways as Deduced from the Genome Sequence2016

    • Author(s)
      Satoshi Kondo, Koichi Hori, Yuko Sasaki-Sekimoto, Atsuko Kobayashi, Tsubasa Kato, Naoko Yuno-Ohta, Takashi Nobusawa, Kinuka Ohtaka, Mie Shimojima, and Hiroyuki Ohta
    • Journal Title

      Front Plant Sci.

      Volume: 7 Pages: 952-952

    • DOI

      10.3389/fpls.2016.00952

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Tangled evolutionary processes with commonality and diversity in plastidial glycolipid synthesis in photosynthetic organisms2016

    • Author(s)
      Hori, K., Nobusawa, T., Watanabe, T., Madoka, Y., Suzuki, H., Shibata, D., Shimojima, M., and Ohta, H.
    • Journal Title

      Biochim. Biophys. Acta, Molecular and Cell Biology of Lipids

      Volume: in press Issue: 9 Pages: 1294-1308

    • DOI

      10.1016/j.bbalip.2016.04.015

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] 車軸藻クレブソルミディウムのゲノムから見た植物の陸上化2016

    • Author(s)
      堀孝一、太田啓之
    • Journal Title

      日本植物学会 BSJ-Review vol. 7B 55-65

      Volume: 7B Pages: 55-65

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Eukaryotic Components Remodeled Chloroplast Nucleoid Organization during the Green Plant Evolution.2015

    • Author(s)
      Kobayashi Y, Takusagawa M, Harada N, Fukao Y, Yamaoka S, Kohchi T, Hori K, Ohta H, Shikanai T, Nishimura Y.
    • Journal Title

      Genome Biol Evol.

      Volume: 8 Issue: 1 Pages: 1-16

    • DOI

      10.1093/gbe/evv233

    • NAID

      120006822487

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 車軸藻植物クレブソルミディウムのオリゴ糖脂質合成酵素の機能解析2018

    • Author(s)
      清水信介,渡邊汀,堀孝一,円由香,下嶋美恵,太田啓之
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ヒメツリガネゴケMADS-box遺伝子は受精に必要な水供給と精子運動を制御する2018

    • Author(s)
      越水静,小藤累美子,佐々木(関本)結子,吉川雅英,下嶋美恵,太田啓之,重信秀治,壁谷幸子,日渡祐二,玉田洋介,村田隆,長谷部光泰
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ゼニゴケMarchantia polymorphaにおけるリン欠乏時の膜脂質転換機構の解析2018

    • Author(s)
      中村将,北浦銀河,佐々木(関本)結子,堀孝一,井雅子,太田啓之,石崎公庸,下嶋美恵
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 車軸藻植物門Klebsormidium flaccidumと陸上植物の細胞壁上に存在する表層脂質層の解析2017

    • Author(s)
      佐々木(関本)結子、近藤智、堀孝一、小林厚子、信澤岳、下嶋美恵、太田啓之
    • Organizer
      第58回日本植物生理学会
    • Place of Presentation
      鹿児島大学
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] 車軸藻クレブソルミディウムの気相、液相培養によるトランスクリプトーム解析2017

    • Author(s)
      堀孝一、太田啓之
    • Organizer
      第58回日本植物生理学会
    • Place of Presentation
      鹿児島大学
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] Comparative transcriptome analysis of Klebsormidium nitens NIES-2285 between solid and liquid culture.2017

    • Author(s)
      Koichi Hori, Hiroyuki Ohta
    • Organizer
      The XIX International Botanical Congress in 2017.
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Primitive auxin response without TIR1 and Aux/IAA in the charophyte alga Klebsormidium nitens2017

    • Author(s)
      Kinuka Ohtaka, Koichi Hori, Yuri Kanno, Mitsunori Seo, Hiroyuki Ohta
    • Organizer
      The XIX International Botanical Congress in 2017.
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Primitive mechanisms to adapt land environments revealed by the genome of a charophyte alga Klebsormidium flaccidum2017

    • Author(s)
      Koichi Hori, Satoshi Kondo, Kinuka Ohtaka, Yuko Sasaki-Sekimoto, Mie Shimojima, Hiroyuki Ohta
    • Organizer
      The XIX International Botanical Congress in 2017.
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Emerging Functions of Land Plant Type Genes in a Charophyte Alga Klebsormidium nitens2017

    • Author(s)
      Hiroyuki Ohta
    • Organizer
      The 65th NIBB Conference Renaissance of Marchantia polymorpha ‐the genome and beyond‐
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Primitive extracellular lipid components on the surface of the Charophytic Alga Klebsormidium flaccidum2016

    • Author(s)
      Satoshi Kondo, Koichi Hori, Yuko Sasaki-Sekimoto, Atsuko Kobayashi, Tsubasa Kato, Naoko Yuno-Ohta, Takashi Nobusawa, Kinuka Ohtaka, Mie Shimojima, Hiroyuki Ohta
    • Organizer
      22nd International Symposium on Plant Lipids
    • Place of Presentation
      Goettingen, Germany
    • Year and Date
      2016-07-03
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Surface lipid remodeling of an aerial alga Klebsormidium flaccidum during land adaptation processes2016

    • Author(s)
      Yuko Sasaki-Sekimoto, Satoshi Kondo, Koichi Hori,, Atsuko Kobayashi, Takashi Nobusawa, Mie Shimojima, Hiroyuki Ohta
    • Organizer
      22nd International Symposium on Plant Lipids
    • Place of Presentation
      Goettingen, Germany
    • Year and Date
      2016-07-03
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Primitive adaptation mechanisms to land environments revealed by the genome of a charophyte alga Klebsormidium flaccidum2016

    • Author(s)
      Satoshi Kondo, Koichi Hori, Yuko Sasaki-Sekimoto, Atsuko Kobayashi, Tsubasa Kato, Naoko Yuno-Ohta, Takashi Nobusawa, Kinuka Ohtaka, Mie Shimojima, Hiroyuki Ohta
    • Organizer
      EMBO workshop New model systems for early land plant evolution
    • Place of Presentation
      Vienna, Austria
    • Year and Date
      2016-06-22
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Revision of Klebsormidium flaccidum Gene models (version 1.1)2016

    • Author(s)
      Koichi Hori, Jyunpei Umetsu, Hiroshi Mori, Ken Kurokawa, Hiroyuki Ohta
    • Organizer
      EMBO workshop New model systems for early land plant evolution
    • Place of Presentation
      Vienna, Austria
    • Year and Date
      2016-06-22
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 車軸藻植物門クレブソルミディウムにおける原始的なオーキシン応答の解析2016

    • Author(s)
      大高きぬ香、堀孝一、太田啓之
    • Organizer
      日本植物生理学会第57回年会
    • Place of Presentation
      岩手大学
    • Year and Date
      2016-03-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] 気生藻類Klebsormidium flaccidumが陸上条件で形成する表層脂質層の解析2016

    • Author(s)
      佐々木(関本) 結子、 近藤智、堀孝一、小林厚子、信澤岳、下嶋美恵、太田啓之
    • Organizer
      日本植物生理学会第57回年会
    • Place of Presentation
      岩手大学
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] 植物の陸上進出と遺伝子発現ネットワークの変遷2016

    • Author(s)
      堀 孝一
    • Organizer
      第5回植物RNAネットワークシンポジウム
    • Place of Presentation
      東京大学駒場キャンパス
    • Year and Date
      2016-01-08
    • Related Report
      2015 Annual Research Report
    • Invited

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Published: 2015-04-16   Modified: 2019-03-29  

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