| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2017: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2015: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
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| Outline of Final Research Achievements |
Sex determination is a critical element in animal development and evolution. In contrast to sex determination based on intrinsic genotypic factors, as commonly seen in many animals, some species show environmental sex determination (ESD), where offspring become male or female in response to an environmental factor(s) during development. We investigated ESD mechanism using American alligator and water flea (daphnia) as a models. In alligator, we identified thermosensitive transient receptor potential (TRP) channels as main initiation candidate. In addition, we revealed that epigenomic factors might be involved in temperature-dependent sex determination. In Daphnids, we identified several juvenile hormone and ecdysone biosynthesis-related genes and investigated their expression levels throughout embryogenesis and molting cycle between male and female. These results provide novel insights towards understanding photoperiod-dependent sex determination process in Daphnids.
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