Molelucalr Mechanisms of Regulating Sperm Function
| Project/Area Number |
15H04398
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Animal physiology/Animal behavior
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Yoshida Manabu 東京大学, 大学院理学系研究科(理学部), 准教授 (60301785)
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| Co-Investigator(Kenkyū-buntansha) |
黒川 大輔 東京大学, 大学院理学系研究科(理学部), 助教 (40342779)
吉田 薫 桐蔭横浜大学, 医用工学部, 准教授 (70398973)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
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| Keywords | 受精 / 精子運動 / 受精能獲得 / 精子走化性 / カルシウムポンプ / カルシウムチャネル / 精子 / シグナル伝達 / カルシウム / 精嚢分泌タンパク質 / CatSper |
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| Outline of Final Research Achievements |
Aim of our research is identify the controlling mechanisms of sperm fertility. In this study, we identify that the seminal plasma protein SVS2, SVS3, and SVS3 mediates fertility of mouse sperm. Interestingly, human seminal plasma protein SEMG1 can also control fertility of mouse sperm.
On the other hand, the sperm-specific Ca2+ channel CatSper is also involved in control of motility and chemotactic behavior of the sperm in the ascidian Ciona intesitinalis. Furthermore, we identified that plasma membrane-type Ca2+/ATPase (PMCA) on the sperm membrane acts as the receptor for sperm attractant and mediates chemotactic behavior.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究は受精成立機構の解明に関わるという性格上、基礎生物学的な成果に留まらず、男性不妊の治療技術や、新たな家畜繁殖技術の開発・改善に寄与するシーズとして発展応用が期待できる。
また、本研究で解析したSVS2は，普遍的に細胞のコレステロール量を調節する試薬として、また、PMCAは細胞内カルシウム濃度を調節するカルシウム拮抗薬としての機能を持ち，創薬のシーズとして発展応用されることが期待できる。
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Report
(4 results)
Research Products
(70 results)
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[Journal Article] Critical roles of seminal plasma on sperm migration in the female reproductive tract2016
Author(s)
Kang, W. Kawano, N., Yamatoya, K., Yoshida, K., Yoshida, M., and Miyado, K.
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Journal Title
Journal of Reproduction Engineering
Volume: 18
Pages: 5-10
Related Report
Peer Reviewed / Open Access
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[Presentation] PMCA and NCKX are expressed, but not involved in activation of sturgeons sperm motility2018
Author(s)
Bondarenko, O., Yoshida, M., Yoshida, K., Ono, C., Dzyuba, B. and Cosson, J.
Organizer
18th International Symposium on Spermatology
Related Report
Int'l Joint Research
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[Presentation] Sperm chemotaxis is regulated by Ca2+ efflux via plasma membrane Ca2+-ATPase2017
Author(s)
Yoshida, K., Shiba, K., Sakamoto, A. Ikenaga, J., Matsunaga, S., Inaba, K., and Yoshida, M.
Organizer
Gordon Research Conference: Fertilization and the Activation of Development
Related Report
Int'l Joint Research
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[Presentation] CatSper has a calcium-permeable voltage sensor domain.2016
Author(s)
Arima, H., Tsutsui, H., Takeshita, K., Nakagawa, A., Yoshida, M., and Okamura, Y.
Organizer
60th Annual Meeting of the Biophysical Society
Place of Presentation
Los Angeles, CA, USA
Year and Date
2016-02-27
Related Report
Int'l Joint Research
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[Presentation] The role of Ca2+ transport and plasma membrane Ca2+-ATPase (PMCA) activity in membrane potential alteration during Bester sperm motility.2015
Author(s)
Bondarenko, O., Yoshida, M., Yoshida, K., Ono, C., Dzyuba, B. and Cosson, J.
Organizer
5th Workshop on the Biology of Fish Gametes
Place of Presentation
Ancona, Italy
Year and Date
2015-09-07
Related Report
Int'l Joint Research
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[Presentation] Seminal vesicle proteins control status of mouse sperm capacitation and prevent ectopic sperm capacitation2015
Author(s)
Araki, N., Kawano, N., Miyado, K, Yoshida, K., and Yoshida, M.
Organizer
Gordon Research Conference: Fertilization and the Activation of Development
Place of Presentation
Holderness School, Plymouth, NH, USA
Year and Date
2015-07-19
Related Report
Int'l Joint Research
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[Presentation] Seminal vesicle protein SVS2 acts as a protectant of sperm sterols and prevents ectopic sperm capacitation2015
Author(s)
Yoshida, M., Araki, N., Kawano, N., MIyado, K, & Yoshida, K.
Organizer
48th Annual Meeting of the Society for the Study of Reproduction
Place of Presentation
Puerto Rico Convention Center, San Juan, Puerto Rico, USA
Year and Date
2015-06-18
Related Report
Int'l Joint Research
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[Book] Flagellar Mechanics and Sperm Guidance2015
Author(s)
J.J. Cosson, A. Guerrero, A. Darszon, C. Wood, C.A. Vines, D.A. Priego-Espinosa, E.H. Smith, E.A. Gaffney, F. Griffin, G. Prokopchuk, G. Martinez-Mekler, G. Cherr, H.A. Guidobaldi, J. Carneiro, K. Inaba, K. Ishimoto, K. Shiba, L.C. Giojalas, M. Yoshida, et al.
Total Pages
426
Publisher
Bentham Science Publishers
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