Elucidation of the working principle of cells based on high dimensional phenotyping
| Project/Area Number |
15H04402
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genetics/Chromosome dynamics
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Ohya Yoshikazu 東京大学, 大学院新領域創成科学研究科, 教授 (20183767)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2017: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2016: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2015: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Keywords | Saccharomyces cerevisiae / morphology / CalMorph / phenotyping / 出芽酵母 / 遺伝子機能 / 形態表現型 / 必須遺伝子 / 形態プロファイル / 形態異常度 / 画像解析 / 遺伝子ネットワーク / 細胞形態 / ケミカルゲノミクス / 高次元表現型解析 / カルシウム / 遺伝的相互作用 / 形態表現型解析 / 遺伝学 / 大規模解析 / ハプロ不全性 |
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| Outline of Final Research Achievements |
It is one of the central issues of genetics to unravel the principle of motion of an organism by genetic information, that is, the principle of how genotype "cause" is linked to phenotype "result". In order to clarify the relationship between genotype and phenotype, we used the excellent genetic tool of unicellular eukaryotic budding yeast (S. cerevisiae) and used cell images obtained with fluorescence microscope. We analyzed the images with image processing system CalMorph and found that there is a close relationship between gene function and morphological phenotype. This was achieved by quantitatively analyzing high dimensional phenotype from two viewpoints including morphological profiling and morphological abnormality. Using that relationship, we succeeded in identifying intracellular targets of drugs.
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Report
(4 results)
Research Products
(43 results)
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[Journal Article] Phenotypic diagnosis of lineage and differentiation during sake yeast breeding2017
Author(s)
Shinsuke Ohnuki, Hiroki Okada, Anne Friedrich, Yoichiro Kanno, Tetsuya Goshima, Hirokazu Hasuda, Masaaki Inahashi, Naoto Okazaki, Hiroyasu Tamura, Ryo Nakamura, Dai Hirata, Hisashi Fukuda, Hitoshi Shimoi, Katsuhiko Kitamoto, Daisuke Watanabe, Joseph Schacherer, Takeshi Akao, Yoshikazu Ohya
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Journal Title
G3 (Bethesda)
Volume: 7
Issue: 8
Pages: 2807-2820
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Systematic analysis of Ca2+ homoeostasis in Saccharomyces cerevisiae based on chemical-genetic interaction profiles2017
Author(s)
Farzan Ghanegolmohammadi, Mitsunori Yoshida, Shinsuke Ohnuki, Yuko Sukegawa, Hiroki Okada, Keisuke Obara, Akio Kihara, Kuninori Suzuki, Tetsuya Kojima, Nozomu Yachie, Dai Hirata, Yoshikazu Ohya
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Journal Title
Mol. Biol. Cell
Volume: 28
Issue: 23
Pages: 3415-3427
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] The Dual Activity Responsible for the Elongation and Branching of β-(1,3)-Glucan in the Fungal Cell Wall.2017
Author(s)
Aimanianda V, Simenel C, Garnaud C, Clavaud C, Tada R, Barbin L, Mouyna I, Heddergott C, Popolo L, Ohya Y, Delepierre M, Latge JP
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Journal Title
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Identification of a mutation causing a defective spindle assembly checkpoint in high ethyl caproate-producing sake yeast strain K1801.2016
Author(s)
Goshima T, Nakamura R, Kume K, Okada H, Ichikawa E, Tamura H, Hasuda H, Inahashi M, Okazaki N, Akao T, Shimoi H, Mizunuma M, Ohya Y, Hirata D.
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Journal Title
Biosci Biotechnol Biochem.
Volume: 80
Issue: 8
Pages: 1657-62
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Plant-derived antifungal agent poacic acid targets β-1,3-glucan2015
Author(s)
Piotrowski JS, Okada H, Lu F, Li SC, Hinchman L, Ranjan A, Smith DL, Higbee AJ, Ulbrich A, Coon JJ, Deshpande R, Bukhman YV, McIlwain S, Ong IM, Myers CL, Boone C, Landick R, Ralph J, Kabbage M, Ohya Y
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Journal Title
Proc Natl Acad Sci U S A
Volume: 112
Issue: 12
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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