| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2017: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2015: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
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| Outline of Final Research Achievements |
To investigate temperature acclimation, we are using cold acclimation in Caenorhabditis elegans. 20°C-cultivated animals did not survival at 2°C. In contrast, 15°C cultivated animals can survive. Previously reported, this cold acclimation is regulated by ASJ temperature sensing-neuron, which releases insulin that is received by intestine and neuron. To identify genes in downstream of insulin-signaling, we performed DNA microarray analysis. We found that expressions of sperm genes were changed in insulin receptor mutants, and sperm mutants showed abnormal cold acclimation. Genetic epistasis analysis suggested that abnormal cold acclimation of sperm mutant was suppressed by the mutations in ASJ. Calcium imaging analysis showed that ASJ neuronal activity in response to temperature was decreased in sperm mutant gsp-4, and rescued by expressing gsp-4 gene in sperm. Thus, we propose a novel feedback between sperm and ASJ temperature sensing-neuron in cold acclimation of C. elgans.
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