Epigenetic regulation of intragenic transposon in plants

• Project/Area Number: 15H04405
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Genetics/Chromosome dynamics
• Research Institution: Okinawa Institute of Science and Technology Graduate University
• Principal Investigator: Saze Hidetoshi 沖縄科学技術大学院大学, 植物エピジェネティクスユニット, 准教授 (70510006)
• Research Collaborator: LE TuNgc 沖縄科学技術大学院大学, 植物エピジェネティクスユニット (30748249) ; Miyazaki Yuji 沖縄科学技術大学院大学, 植物エピジェネティクスユニット (40708773)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000); Fiscal Year 2017: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2016: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2015: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
• Keywords: エピジェネティクス / トランスポゾン / 植物ゲノム / 植物遺伝学

Outline of Final Research Achievements

Genomes of vertebrates and plants contain a substantial number of transposable elements (TEs), which are often inserted in genic regions. In this study, we aimed to analyze genome-wide distribution of transposable elements in the model plant Arabidopsis thaliana. We showed that about 3% of TEs are intragenic, especially are inserted in intronic regions. Further study showed that epigenetic silencing of intronic repeats requires RNA-directed DNA methylation pathway, MET1 and DDM1.

Research Products

• [Journal Article] Transposon-driven environmental adaptation in plants (2018)
  • Author(s): 佐瀬英俊
  • Journal Title: Leading Author's Volume: 7 Pages: e001
  • DOI: 10.7875/leading.author.7.e001
  • Peer Reviewed / Open Access
• [Journal Article] Epigenetic regulation of intronic transgenes in Arabidopsis (2017)
  • Author(s): Osabe K, Harukawa Y, Miura S, Saze H.
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 45166-45166
  • DOI: 10.1038/srep45166
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A stress-Activated Transposon in Arabidopsis Induces transgenerational Abscisic Acid Insensitivity. Scientific Reports. (2016)
  • Author(s): Ito H, Kim JM, Matsunaga W, Saze H, Matsui A, Endo TA, Harukawa Y, Takagi H, Yaegashi H, Masuta Y, Masuda S, Ishida J, Tanaka M, Takahashi S, Morosawa T, Toyoda T, Kakutani T, Kato A, Seki M.
  • Journal Title: Scientific Reports Volume: 6, Article number: 23181 Issue: 1 Pages: 1-12
  • DOI: 10.1038/srep23181
  • Peer Reviewed / Open Access
• [Journal Article] Epigenetic regulation of intragenic transposable elements impacts gene transcription in Arabidopsis thaliana. (2015)
  • Author(s): Le Tu N., Miyazaki Y., Takuno S., Saze H.
  • Journal Title: Nucleic Acids Research Volume: 43 Issue: 8 Pages: 3911-21
  • DOI: 10.1093/nar/gkv258
  • Peer Reviewed / Open Access
• [Journal Article] DNA Methylation within Transcribed Regions. (2015)
  • Author(s): To TK, Saze H, Kakutani T
  • Journal Title: Plant Physiol Volume: 168 Issue: 4 Pages: 1219-1225
  • DOI: 10.1104/pp.15.00543
  • Peer Reviewed / Open Access
• [Presentation] Epigenetic regulation of intragenic transposons impacts gene transcription in Arabidopsis. (2017)
  • Author(s): Hidetoshi Saze
  • Organizer: Cutting Edge Developments in RNA biology for the Control of Gene Expression
  • Int'l Joint Research / Invited
• [Presentation] Epigenetic regulation of intronic repeats in plants (2017)
  • Author(s): Hidetoshi Saze
  • Organizer: Taiwan-Japan Plant Biology 2017
  • Int'l Joint Research / Invited
• [Presentation] Epigenetic regulation of genes and transposable elements in plants (2017)
  • Author(s): Hidetoshi Saze
  • Organizer: The 33rd Annual Meeting of the Society of Population Ecology
  • Invited
• [Presentation] 植物ゲノムにおける遺伝子内反復配列のエピジェネティック制御 (2016)
  • Author(s): 佐瀬英俊
  • Organizer: 日本遺伝学会第88会年会
  • Place of Presentation: 日本大学国際関係学部（静岡県三島市）
• [Presentation] Epigenetic Regulation of Repeats in Intronic Region in the Arabidopsis thaliana genome (2015)
  • Author(s): 佐瀬英俊
  • Organizer: 日本エピジェネティクス研究会
  • Place of Presentation: 学術総合センター
  • Year and Date: 2015-05-25