| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2015: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Outline of Final Research Achievements |
We experimentally verified whether it is possible to artificially acquire cis-elements in the distal region of the genomic endogenous toolkit gene and cause gene expression evolution. When ectopic enhancer knock-in mice were generated using the Dlx3/4 gene cluster as a model system, Dlx3/4 gene expression was upregulated by the inserted enhancer. This result indicated that target gene expression change could be realized by knocking in the ectopic enhancer at appropriate genomic position. In addition, we have established an efficient genome editing protocol for knock-in.
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