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Evolution of host cercozoans toward the establishment of chlorarachniophytes

Research Project

Project/Area Number 15H04411
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biodiversity/Systematics
Research InstitutionUniversity of Tsukuba

Principal Investigator

ISHIDA Ken-ichiro  筑波大学, 生命環境系, 教授 (30282198)

Co-Investigator(Renkei-kenkyūsha) NAKAYAMA Takeshi  筑波大学, 生命環境系, 准教授 (40302369)
Research Collaborator SHIRATORI Takashi  
SUZUKI Shigekatsu  
KATO Yuta  
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2017: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2016: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2015: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Keywords進化 / 二次共生 / Minorisa / 原生生物 / 系統分類 / 藻類 / クロララクニオン藻 / ケルコゾア / 植物 / Rhabdamoeba / 分類 / 環境DNA
Outline of Final Research Achievements

The chlorarachniophytes acquired their plastids from a green alga via a secondary endosymbiosis. To know the evolution of ancestral host-related organisms before the plastid acquisition, the colorless cercozoans that are closely related to the chlorarachniophytes were searched and 5 new cultured strains were established, and new findings on the phylogeny, taxonomy and evolution were obtained from morphological and ultrastructureal observantions and RNAseq analyses. The present research revealed that there are 3 clades of cercozoans that are closely related to the chlorarachniophytes and that the acquisition of plastid via the secondary endosymbiosis occurred at the ancestor of chlorarachniophytes after diverged from those sister cercozoans. The new findings on the evolution of flagellar apparatus were also provided. The presence of three new species of Minorisa, the closest colorless relative of chlorarachniophytes, were also suggested.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (22 results)

All 2017 2016 2015 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Open Access: 1 results,  Acknowledgement Compliant: 3 results) Presentation (11 results) (of which Int'l Joint Research: 4 results,  Invited: 1 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Viridiuvalis adhaerens gen. et sp. nov., a novel colony-forming chlorarachniophyte.2017

    • Author(s)
      Takashi Shiratori, Sakuya Fujita, Tenma Shimizu, Takeshi Nakayama, Ken-ichiro Ishida
    • Journal Title

      Journal of Plant Research

      Volume: 130 Issue: 6 Pages: 999-1012

    • DOI

      10.1007/s10265-017-0961-1

    • NAID

      40021364870

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A New Heterotrophic Cryptomonad: Hemiarma marina n. g., n. sp..2016

    • Author(s)
      Takashi Shiratori, Ken-ichiro Ishida
    • Journal Title

      Journal of Eukaryotic Microbiology

      Volume: 63(6) Issue: 6 Pages: 804-812

    • DOI

      10.1111/jeu.12327

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Diurnal Transcriptional Regulation of Endosymbiotically Derived Genes in the Chlorarachniophyte Bigelowiella natans.2016

    • Author(s)
      Shigekatsu Suzuki, Ken-Ichiro Ishida, Yoshihisa Hirakawa
    • Journal Title

      Genome Biology and Evolution

      Volume: 8 Issue: 9 Pages: 2672-2682

    • DOI

      10.1093/gbe/evw188

    • NAID

      120007129385

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Plastid genome sequences of Gymnochlora stellata, Lotharella vacuolata, and Partenskyella glossopodia reveal remarkable structural conservation among chlorarachniophyte species.2016

    • Author(s)
      Suzuki S, Hirakawa Y, Kofuji R, Sugita M, Ishida K.
    • Journal Title

      Journal of Plant Research

      Volume: 印刷中 Issue: 4 Pages: 581-590

    • DOI

      10.1007/s10265-016-0804-5

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Fine-structural observations on siliceous scale production and shell assembly in the testate amoeba Paulinella chromatophora.2016

    • Author(s)
      Mami Nomura, Ken-ichiro Ishida
    • Journal Title

      Protist

      Volume: 167(4) Issue: 4 Pages: 303-318

    • DOI

      10.1016/j.protis.2016.05.002

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Entamoeba marina n. sp.; a New Species of Entamoeba isolated from tidal flat sediment of Iriomote Island, Okinawa, Japan2016

    • Author(s)
      Takashi Shiratori, Ken-Ichiro Ishida
    • Journal Title

      The Journal of Eukaryotic Microbiology

      Volume: 印刷中 Issue: 3 Pages: 1-7

    • DOI

      10.1111/jeu.12276

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Trachyrhizium urniformis n. g., n. sp., a novel marine filose thecate amoeba related to a cercozoan environmental clade (novel clade 4)2016

    • Author(s)
      Takashi Shiratori, Ken-Ichiro Ishida
    • Journal Title

      The Journal of Eukaryotic Microbiology

      Volume: 印刷中 Issue: 6 Pages: 1-10

    • DOI

      10.1111/jeu.12319

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] A new deep-branching stramenopile, Platysulcus tardus gen. nov., sp. nov.2015

    • Author(s)
      Takashi Shiratori, Takeshi Nakayama, Ken-ichiro Ishida
    • Journal Title

      Protist

      Volume: 166 Pages: 337-348

    • NAID

      120007134801

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Presentation] クロララクニオン藻に近縁な無色ケルコゾア生物の分類学的研究2017

    • Author(s)
      加藤 雄大、白鳥 峻志、石田 健一郎
    • Organizer
      日本藻類学会第41 回大会
    • Place of Presentation
      高知大学、高知県・高知市
    • Year and Date
      2017-03-23
    • Related Report
      2016 Annual Research Report
  • [Presentation] Phylogeny and Evolution of Basal Stramenopiles.2017

    • Author(s)
      Rabindra Thakur, Takashi Shiratori, Ken-ichiro Ishida
    • Organizer
      日本藻類学会第41 回大会
    • Place of Presentation
      高知大学、高知県・高知市
    • Year and Date
      2017-03-23
    • Related Report
      2016 Annual Research Report
  • [Presentation] クロララクニオン藻に近縁な無色ケルコゾア生物の分類学的研究2017

    • Author(s)
      加藤雄大、白鳥峻志、石田健一郎
    • Organizer
      第50回日本原生生物学会大会 第1回日本共生生物学会大会合同大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Morphology, ultrastructure and phylogeny of a new species of Glissandra (Protista incertae sedis)2017

    • Author(s)
      Takashi Shiratori, Euki Yazaki, Yuji Inagaki, Ken-ichiro Ishida
    • Organizer
      15th international congress of protistology, Prague, Czech Republic
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 捕食能をもつ新奇バクテリアの報告2017

    • Author(s)
      白鳥 峻志、鈴木 重勝、石田 健一郎
    • Organizer
      第50回日本原生生物学会大会 第1回日本共生生物学会大会合同大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] クロララクニオン藻の遺伝子発現から紐解く色素体制御機構2016

    • Author(s)
      平川泰久, 鈴木重勝, 石田健一郎
    • Organizer
      日本植物学会第80回沖縄大会
    • Place of Presentation
      沖縄コンベンションセンター、沖縄県・宜野湾市
    • Year and Date
      2016-09-16
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 有殻アメーバの被殻構築から探る細胞機能の可能性2016

    • Author(s)
      野村真未、石田健一郎
    • Organizer
      日本植物学会第80回沖縄大会
    • Place of Presentation
      沖縄コンベンションセンター、沖縄県・宜野湾市
    • Year and Date
      2016-09-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] クロララクニオン藻のオルガネラDNA ポリメラーゼの進化2016

    • Author(s)
      渡辺ありさ, 鈴木重勝, 平川泰久, 石田健一郎
    • Organizer
      日本植物学会第80回沖縄大会
    • Place of Presentation
      沖縄コンベンションセンター、沖縄県・宜野湾市
    • Year and Date
      2016-09-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] Identification and transcriptome analysis of a non-photosynthetic eukaryovorous amoeba phylogenetically related to chlorarachniophytes.2016

    • Author(s)
      Shigekatsu Suzuki, Takashi Shiratori, Ken-ichiro Ishida
    • Organizer
      ICES 2016, Kyoto
    • Place of Presentation
      Kyoto Prefectural University, Kyoto, Japan
    • Year and Date
      2016-09-10
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Evolution of organellar DNA polymerases in Chlorarachniophyte algae.2016

    • Author(s)
      Arisa Watanabe, Shigekatsu Suzuki, Yoshihisa Hirakawa, Ken-ichiro Ishida
    • Organizer
      ICES 2016, Kyoto
    • Place of Presentation
      Kyoto Prefectural University, Kyoto, Japan
    • Year and Date
      2016-09-10
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Organelle DNA replication in chlorarachniophyte algae.2016

    • Author(s)
      Yoshihisa Hirakawa, Arisa Watanabe, Shigekatsu Suzuki, Ken-ichiro Ishida
    • Organizer
      Protist 2016
    • Place of Presentation
      Moscow, Russia
    • Year and Date
      2016-06-06
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Book] 共生微生物 ~生物と密接に関わるミクロな生命体~2016

    • Author(s)
      石田健一郎(大野博司 編)
    • Publisher
      株式会社化学同人
    • Related Report
      2016 Annual Research Report
  • [Book] 進化の謎をゲノムで解く2015

    • Author(s)
      石田健一郎(監修:長谷部光泰)
    • Total Pages
      9
    • Publisher
      秀潤社
    • Related Report
      2015 Annual Research Report
  • [Remarks] コンブや珪藻などを含む大系統群の根元に位置する新種の生物を発見

    • URL

      http://www.tsukuba.ac.jp/attention-research/r201506191200.html

    • Related Report
      2015 Annual Research Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

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