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Conservation of biodiversity hotspots by taking advantage of genomic information and ex situ populations

Research Project

Project/Area Number 15H04414
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biodiversity/Systematics
Research InstitutionKyoto University

Principal Investigator

Isagi Yuji  京都大学, 農学研究科, 教授 (50325130)

Co-Investigator(Kenkyū-buntansha) 邑田 仁  東京大学, 大学院理学系研究科(理学部), 教授 (90134452)
Research Collaborator KOMAKI Yoshiteru  東京大学, 大学院理学系研究科
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2017: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2016: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Keywords保全ゲノミクス / 絶滅危惧種 / 固有種 / 種分化 / 隠蔽種 / 小笠原諸島 / 生育域外保全 / 国内希少野生動植物種 / 生物多様性ホットスポット / 生物多様性保全 / 空間的遺伝構造 / RAD-seq / RNA-seq / トランスクリプトーム解析 / 縮約ゲノム解読 / 種の保存法
Outline of Final Research Achievements

We conducted analyses of conservation genomics on wild and ex situ individuals of critically endangered endemic plant species of the Bonin Islands. Using genomic information from RAD-seq and RNA-seq, genetic diversity, genetic differentiations, spatial genetic structure, intraspecies phylogeny and genes selected during radiative speciation for endangered endemics were inferred. We found species specific genetic traits for each taxon, such that, species without any genetic diversity in both wild and ex situ populations, one with significantly accumulated deteriorated genes and those under processes of speciation containing cryptic species. These findings are useful to achieve appropriate biological conservation activities.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (15 results)

All 2018 2017 2016

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (12 results) (of which Int'l Joint Research: 4 results,  Invited: 4 results)

  • [Journal Article] Development of microsatellite markers for an endemic shrub, Leptopetalum grayi (Rubiaceae), on the Ogasawara Islands using Ion Torrent next-generation sequencing.2016

    • Author(s)
      Tsuda Y, Nakahama N, Izuno A, Kyogoku D, Arima K, Kato H, Isagi Y
    • Journal Title

      Conservation Genetics Resources

      Volume: 8 Issue: 4 Pages: 587-594

    • DOI

      10.1007/s12686-016-0635-7

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Twenty novel microsatellite primers in critically endangered Melastoma tetramerum var. tetramerum (Melastomataceae).2016

    • Author(s)
      Narita A, Izuno A, Komaki Y, Tanaka T, Murata J, Isagi Y
    • Journal Title

      Applications in Plant Sciences

      Volume: 4 Issue: 9 Pages: 1-4

    • DOI

      10.3732/apps.1600053

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Two New Combinations of Leptopetalum (Rubiaceae) Based on Molecular Phylogenetic Evidence.2016

    • Author(s)
      Naiki A, Komaki Y, Mizunashi K,Ohi-Toma T
    • Journal Title

      Journal of Japanese Botany

      Volume: 91 Pages: 337-344

    • NAID

      40021036699

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Presentation] 海外に多個体が生育する国内希少野生動植物種の保全ゲノミクス2018

    • Author(s)
      芝林真友, 栗田和, 横田昌嗣, 阿部篤志, 赤井賢成, 國府方吾郎, 長澤淳一, 志内利明, 市河三英, 橋本季正, 遊川知久, 阪口翔太, 寺峰孜, 井鷺裕司
    • Organizer
      日本植物分類学会第17回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 小笠原諸島の希少植物タイヨウフウトウカズラの実生の遺伝的多様性 -SSR/MIG-seq による解析と比較2017

    • Author(s)
      成田あゆ、兼子伸吾、陶山佳久、綱本良啓、小牧義輝、葉山佳代、坂入祐子、井鷺裕司
    • Organizer
      第64回日本生態学会大会
    • Place of Presentation
      東京都、早稲田大学
    • Year and Date
      2017-03-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] 小笠原諸島に生育する絶滅危惧固有植物の保全ゲノミクス2017

    • Author(s)
      井鷺裕司、兼子伸吾、成田智史、木下豪太、成田あゆ、永野淳、手塚あゆみ、八杉公基、鈴木節子、加藤英寿、加藤朗子、須貝杏子
    • Organizer
      第64回日本生態学会大会
    • Place of Presentation
      東京都、早稲田大学
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] 網羅的遺伝解析に基づく種の保存法対象種の保全2017

    • Author(s)
      井鷺裕司、京極大助、田畑諒一、横田昌嗣、阿部篤志
    • Organizer
      第16回植物分類学会大会
    • Place of Presentation
      京都市、京都大学
    • Year and Date
      2017-03-11
    • Related Report
      2016 Annual Research Report
  • [Presentation] Conservation genetics with information from NGS in the Bonin Islands, a UNESCO World Heritage site.2017

    • Author(s)
      Isagi Y, Kaneko S, Narita A, Suzuki S, Ando H, Kato H
    • Organizer
      The 48th Annual Meeting for the Korean Society of Plant Taxonomists, International Symposium "Island Plants - Evolution and Conservation"
    • Place of Presentation
      Suwon, Korea
    • Year and Date
      2017-02-08
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Conservation genetics/genomics on remote oceanic island ecosystems with information from NGS2017

    • Author(s)
      Isagi Y, Kaneko K, Narita A, Suzuki S, Ando H, Kato H
    • Organizer
      International Academic Conference on the Formation Mechanism of Plant Diversity in East Asia and Conservation of Endangered Plants
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 遺伝的地域性の保全の必要性2016

    • Author(s)
      井鷺裕司
    • Organizer
      第47回日本緑化工学会大会
    • Place of Presentation
      京都市、稲盛記念会館
    • Year and Date
      2016-10-01
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] NGSによる大量塩基情報解読を活用した希少植物保全2016

    • Author(s)
      井鷺裕司
    • Organizer
      日本植物学会第80回大会
    • Place of Presentation
      宜野湾市、沖縄コンベンションセンター
    • Year and Date
      2016-09-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] Conservation genetics with information from NGS in the Bonin Islands, a UNESCO World Heritage site.2016

    • Author(s)
      Isagi Y, Kaneko S, Narita S, Narita A, Ando H, Komura T
    • Organizer
      The 7th EAFES (East Asian Federation of Ecological Societies) International Congress
    • Place of Presentation
      Daegu, Korea
    • Year and Date
      2016-04-19
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ゲノムワイドSNPを用いた小笠原諸島ムラサキシキブ属の種分化における遺伝的背景の解明2016

    • Author(s)
      成田智史、鈴木節子、加藤英寿、井鷺裕司
    • Organizer
      日本生態学会第63回大会
    • Place of Presentation
      仙台国際センター
    • Year and Date
      2016-03-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] 小笠原諸島における送粉生態系攪乱が固有種シマザクラの繁殖成功と遺伝的特徴に与える影響 ー対照的な2島の比較解析ー2016

    • Author(s)
      津田優一、加藤英寿、井鷺裕司
    • Organizer
      日本生態学会第63回大会
    • Place of Presentation
      仙台国際センター
    • Year and Date
      2016-03-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] Conservation genetics with information from NGS in the Bonin Islands, a UNESCO World Heritage site2016

    • Author(s)
      Yuji Isagi, Shingo Kaneko, Satoshi Narita, Ayu Narita, Haruko Ando, Taketo Komura
    • Organizer
      The 13th International Symposium on Integrated Field Science
    • Place of Presentation
      Graduate School of Agriculture, Tohoku University
    • Year and Date
      2016-03-10
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited

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Published: 2015-04-16   Modified: 2019-03-29  

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