| Budget Amount *help |
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2017: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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| Outline of Final Research Achievements |
Zea nicaraguensis, which is a wild relative of maize (Z. mays ssp. mays), has higher tolerance of soil waterlogging than maize. We found that Z. nicaraguensis (but not maize) can form a barrier to radial oxygen loss (ROL). To identify a locus controlling ROL barrier formation in Z. nicaraguensis, we used a library of introgression lines (ILs), each containing a chromosome segment from Z. nicaraguensis in the maize inbred line Mi29. By fine-mapping and phenotyping, we discovered that the gene controlling ROL barrier formation was located on about 232 kb region of chromosome 3. We conducted gene expression analyses and then finally found one candidate gene. We also carried out suberin staining of root cross sections. However, we were not able to find any relationship between suberin staining pattern and root ROL barrier formation.
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