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Epigenetic regulation of root phenotypic plasticity contributing drought avoidance in rice

Research Project

Project/Area Number 15H04435
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Science in genetics and breeding
Research InstitutionNagoya University

Principal Investigator

Inukai Yoshiaki  名古屋大学, 農学国際教育協力研究センター, 准教授 (20377790)

Co-Investigator(Kenkyū-buntansha) 兒島 孝明  名古屋大学, 生命農学研究科, 講師 (40509080)
高橋 実鈴  名古屋大学, 生命農学研究科, 特任助教 (20738091)
Co-Investigator(Renkei-kenkyūsha) KOJIMA Takashi  名古屋大学, 生命農学研究科, 講師 (40509080)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2017: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2016: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Keywordsイネ / 根系形成 / 分子機構 / 根系形態 / ストレス応答 / 根系形成機構 / ヒストン修飾 / 遺伝子発現 / 突然変異 / 育種学 / 遺伝学 / 遺伝子 / ゲノム / ストレス / 植物遺伝育種学 / エピジェネティクス
Outline of Final Research Achievements

Development of lateral roots (LRs) is promoted when parental root elongation is suppressed. Promotion of LR growth compensates for parental root growth, which contributes to the maintenance of total root length. However, the developmental
processes underlying the compensatory growth of LRs are still unclear. In this study, we induced LR compensatory growth in rice by surgical excision of a parent root tip, and analyzed the morphological and anatomical changes in LRs. Our analysis revealed that the morphological and anatomical changes of LRs that are induced by the root-tip excision of the parent vary continuously, and are dependent on both the developmental stage of LR primordia at the site of root-cutting and the distance from the cut site. Then, by using this method, we also tried to understand the important genes that regulate LR development epigenetically through ChIP-Seq analysis with Histone H3K27me3 antibody.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (16 results)

All 2018 2017 2016 2015

All Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (14 results) (of which Int'l Joint Research: 5 results,  Invited: 2 results)

  • [Journal Article] OsPIN2, which encodes a member of the auxin efflux carrier proteins, is involved in root elongation growth and lateral root formation patterns via the regulation of auxin distribution in rice2018

    • Author(s)
      Inahashi Hiroki、Shelley Israt Jahan、Yamauchi Takaki、Nishiuchi Shunsaku、Takahashi-Nosaka Misuzu、Matsunami Maya、Ogawa Atsushi、Noda Yusaku、Inukai Yoshiaki
    • Journal Title

      Physiologia Plantarum

      Volume: - Issue: 2 Pages: 216-225

    • DOI

      10.1111/ppl.12707

    • NAID

      120006554484

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Analysis of the rrl3 Mutants Reveals the Importance of Arginine Biosynthesis in the Maintenance of Root Apical Meristem in Rice2017

    • Author(s)
      Shelley Israt J.、Watanabe Sayaka、Ozaki Hiroaki、Nagasawa Nobuhiro、Ogawa Atsushi、Takahashi-Nosaka Misuzu、Nishiuchi Shunsaku、Yamauchi Akira、Kitano Hidemi、Inukai Yoshiaki
    • Journal Title

      Journal of Plant Studies

      Volume: 7 Issue: 1 Pages: 36-36

    • DOI

      10.5539/jps.v7n1p36

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] イネの根端切除時における側根形態の可塑的反応機構の解析2018

    • Author(s)
      河合翼、兒島孝明、山内章、犬飼義明
    • Organizer
      第133回日本育種学会講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] イネにおける種子根の根端切除が側根形成に及ぼす影響2018

    • Author(s)
      河合翼、兒島孝明、山内章、犬飼義明
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Compensatory growth of lateral roots responding to excision of seminal root tip in rice2018

    • Author(s)
      Kawai, T., Nosaka-Takahashi, M., Yamauchi, A. and Inukai, Y.
    • Organizer
      2017 Symposium on “Root Biology”
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Involvement of H3K27 methyltransferase complex in the regulation of phenotypic plasticity in lateral root development2018

    • Author(s)
      Nonawin, B. L., Nosaka-Takahashi, M., Kawai, T., Yamauchi, A. and Inukai, Y.
    • Organizer
      2017 Symposium on “Root Biology”
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] イネにおける種子根の根端切除に応答した側根の補償生長2017

    • Author(s)
      河合 翼・高橋 ( 野坂) 美鈴・山内 章・犬飼 義明
    • Organizer
      日本育種学会第131回講演会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2017-03-29
    • Related Report
      2016 Annual Research Report
  • [Presentation] イネ種子根におけるOSHB 遺伝子の発現解析2017

    • Author(s)
      高橋(野坂) 実鈴・佐藤(永澤)奈美子・桧原 健一郎・犬飼 義明
    • Organizer
      日本育種学会第131回講演会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2017-03-29
    • Related Report
      2016 Annual Research Report
  • [Presentation] Trial of rice breeding for drought avoidance using the vrs1 gene that promotes lateral root development2016

    • Author(s)
      Hasegawa, T., Shibata, A., Takahashi-Nosaka, M., Nishiuchi, S., Samejima, H., Wainaina, C., Menge, D., Kikuta, M., Yamauchi, A., Makihara, D. and Inukai, Y.
    • Organizer
      SATREPS International Symposium, "Tailor-made rice breeding and cultivation technology development for sub-Saharan Africa;Progress and future prospects of the SATREPS project in Kenya"
    • Place of Presentation
      Nairobi, Kenya
    • Year and Date
      2016-12-06
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] イネにおける種子根の根端近傍切除が側根の形態に及ぼす影響2016

    • Author(s)
      河合翼・山内章・犬飼義明
    • Organizer
      第45回根研究集会
    • Place of Presentation
      岡山大学
    • Year and Date
      2016-09-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] 土壌水分・窒素濃度とイネの根の成長2016

    • Author(s)
      犬飼義明
    • Organizer
      第2回植物の栄養研究会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2016-09-02
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] イネ種子根におけるOsHB転写産物の解析2016

    • Author(s)
      高橋(野坂)実鈴・佐藤(永澤)奈美子・桧原健一郎・犬飼義明
    • Organizer
      イネ遺伝学・分子生物学ワークショップ2016
    • Place of Presentation
      名古屋大学
    • Year and Date
      2016-07-04
    • Related Report
      2016 Annual Research Report
  • [Presentation] Effect of nitrogen, osmotic stress and sugar content to the lateral root development in rice2016

    • Author(s)
      Lucob, N. B., Takahashi-Nosaka, W. and Inukai, Y.
    • Organizer
      第24回育種学会中部地区談話会
    • Place of Presentation
      農研機構 野菜花き研究部門、三重県
    • Related Report
      2016 Annual Research Report
  • [Presentation] A Histone H3 Lysine-27 Methyltransferase Complex is involved in lateral root development in rice2015

    • Author(s)
      Nonawin B. Lucob, Misuzu Takahashi-Nosaka, Akihide Shibata, Hiroaki Inahashi, Akira Yamauchi1 and Yoshiaki Inukai
    • Organizer
      第23回育種学会中部地区談話会
    • Place of Presentation
      愛知県長久手町
    • Year and Date
      2015-11-21
    • Related Report
      2015 Annual Research Report
  • [Presentation] Developmental and functional responses of rice root system to soil water and nutrient conditions2015

    • Author(s)
      Yoshiaki Inukai, Misuzu Takahashi-Nosaka, Cornelius M. Wainaina, Akihide Shibata, Nonawin B. Lucob, Hiroki Inahashi, Mana. Kano-Nakata, Emi Kameoka, Roel R. Suralta, Jonathan M. Niones, Thiem T. Tran, Stella O. Nketia, Israt J. Shelley, Yuka Kitomi, Satomi Ohashi, Shunsaku Nishiuchi, Hidemi Kitano and Akira Yamauchi
    • Organizer
      9th Symposium for International Society of Root Research
    • Place of Presentation
      Canberra, Australia
    • Year and Date
      2015-10-06
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Molecular mechanisms of phenotypic plasticity for lateral root development in rice2015

    • Author(s)
      Nonawin B. Lucob, Akihide Shibata, Misuzu Takahashi-Nosaka, Hiroki Inahashi, Yoshiaki Inukai
    • Organizer
      9th Symposium for International Society of Root Research
    • Place of Presentation
      Canberra, Australia
    • Year and Date
      2015-10-06
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research

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Published: 2015-04-16   Modified: 2019-03-29  

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