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Linkage map construction based on LD and its application to polyploid species

Research Project

Project/Area Number 15H04441
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Science in genetics and breeding
Research InstitutionKazusa DNA Research Institute

Principal Investigator

Sachiko Isobe  公益財団法人かずさDNA研究所, 先端研究部, 室長 (20343973)

Co-Investigator(Kenkyū-buntansha) 中谷 明弘  大阪大学, 医学系研究科, 特任教授(常勤) (60301149)
田中 勝  国立研究開発法人農業・食品産業技術総合研究機構, 九州沖縄農業研究センター, グループ長 (60391455)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2017: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2016: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2015: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Keywords連鎖地図 / 高次倍数性 / 連鎖不平衡 / ゲノム配列 / 育種学 / 遺伝学
Outline of Final Research Achievements

Linkage analysis was performed with a S1 population of strawberry derived from a Japanese variety, ‘Reiko’, a S1 population of sweetpotato derived from a Chinese variety, ‘Xushu 18’, and a F1 population cross between ‘Xhushu 18’ and an Ipomoea trifida accession, ‘K123-11’.
Although linkage maps were successfully constructed in the S1 populations of strawberry and sweetpotato, it was difficult to generate linkage groups in the sweetpotato F1 population with typical approach in linkage map construction. A sofrtware was developed for generation on linkage groups based on linkage disequilibrium (LD) between the two loci. The software calculates indexes representing LDs between the all combinations of two loci and constructing hierarchic structure based on the LD indexes.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (18 results)

All 2017 2016 2015 Other

All Int'l Joint Research (2 results) Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (14 results) (of which Int'l Joint Research: 9 results,  Invited: 7 results)

  • [Int'l Joint Research] RDA Nat Inst of Agric Sci/KRIBB(韓国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Institute of Sweetpotato Research, CAAS/China Agricultural University(中国)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Discrimination of candidate subgenome-specific loci by linkage map construction with an S1 population of octoploid strawberry (Fragaria x ananassa)2017

    • Author(s)
      Nagano S, Shirasawa K, Hirakawa H, Maeda F, Ishikawa M, Isobe SN
    • Journal Title

      BMC Genomics

      Volume: 18 Issue: 1 Pages: 374-374

    • DOI

      10.1186/s12864-017-3762-y

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 3.A high-density SNP genetic map consisting of a complete set of homologous groups in autohexaploid sweetpotato (Ipomoea batatas).2017

    • Author(s)
      Shirasawa K, Tanaka M, Takahata Y, Ma D, Cao Q, Liu Q, Zhai H, Kwak SS, Jeong JC, Yoon UH, Lee HU, Hirakawa H, Isobe S
    • Journal Title

      Nature Scientific Report

      Volume: 7 Issue: 1 Pages: 44207-44207

    • DOI

      10.1038/srep44207

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] 異質八倍体栽培イチゴゲノムのフェージング配列の染色体への位置付けと祖先ゲノムの推定2017

    • Author(s)
      白澤健太、平川英樹、寺地真由子、永野聡一郎、長崎英樹、前田ふみ、柳 智博、磯部祥子
    • Organizer
      日本育種学会第131回講演会
    • Place of Presentation
      名古屋大学、愛知
    • Year and Date
      2017-03-29
    • Related Report
      2016 Annual Research Report
  • [Presentation] A high-density SNP linkage map toward whole genome sequencing in autohexaploid sweetpotato2017

    • Author(s)
      Shirasawa K, Tanaka M, Takahata Y, Ma D, Cao Q, Liu Q, Zhai H, Kwak S, Jeong J, Yoon U, Lee H, Hirakawa H, Isobe S
    • Organizer
      PAG Asia
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] De novo whole genome assembly in allo-octoploid strawberry2017

    • Author(s)
      Shirasawa K, Nagano S, Hirakawa H, Nagasaki H, Maeda F, Isobe S
    • Organizer
      XIX international botanial cogress
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Sweetpotato genome sequencing under TRAS collaboration2017

    • Author(s)
      TRAS Sweetpotato genome sequencing consortium (Presenter: Isobe S)
    • Organizer
      XIX international botanial cogress
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 白澤健太, 長崎英樹, 田中 勝, 岡田吉弘, 高畑康浩, 平川英樹, 磯部祥子2017

    • Author(s)
      同質六倍体サツマイモ栽培種のゲノム解読に向けた取り組み
    • Organizer
      日本植物学会第81回大会シンポジウム
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] サツマイモ塊根におけるGWASを用いた着色ゲノム領域の探索2017

    • Author(s)
      木村拓海、田中勝、白澤健太、磯部祥子、田原誠、門田有希
    • Organizer
      日本育種学会第133回講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] De novo Whole Genome Assembly in AlloOctoploid Strawberry2017

    • Author(s)
      Shirasawa K, Nagano S, Hirakawa H, Nagasaki H, Maeda F, Isobe S
    • Organizer
      Plant and Animal Genome XXV
    • Place of Presentation
      San Diego, USA
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Genetic map with SNPs from ddRAD-Seq analysis in sweetpotato2016

    • Author(s)
      Shirasawa K, Tanaka M, Takahata Y, Ma D, Cao Q, Liu Q, Zhai H, Kwak SS, Jeong JC, Yoon UH, Hirakawa H, Isobe S
    • Organizer
      7th Internatinal Sweetpotato Workshop
    • Place of Presentation
      済南、中国
    • Year and Date
      2016-10-10
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 同質六倍体で2n=6x=90のゲノムを持つサツマイモ栽培種の高密度SNP遺伝地図の作成.2016

    • Author(s)
      白澤健太、田中勝、高畑康浩、Ma D、Caom Q、Liu Q、Zhai H、Kwak SS、Jeong JC、Yoon UH、平川英樹、磯部祥子
    • Organizer
      日本育種学会第130回講演会
    • Place of Presentation
      鳥取大学、鳥取
    • Year and Date
      2016-09-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] Hirakawa H, Shirasawa K, Nagano S, Nagasaki H, Isobe S2016

    • Author(s)
      Genome sequencing and SNP discovery in the octoploid strawberry
    • Organizer
      VIII International Strawberry Symposium
    • Place of Presentation
      Qubec City, Canada
    • Year and Date
      2016-08-14
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Identification of chromosome specific regions in 8X cultivated strawberry by construction of a SNP map.2016

    • Author(s)
      Soichiro Nagano, Kenta Shirasawa, Hideki Hirakawa, Fumi Maeda, Masami Ishikawa and Sachiko Isobe
    • Organizer
      XXIV Plant and Animal Genome conference
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2016-01-10
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] トマトにおけるRAD-Seq法の効率的な利用法の検討と高密度連鎖地図の作成2015

    • Author(s)
      白澤健太、平川英樹、磯部祥子
    • Organizer
      園芸学会
    • Place of Presentation
      徳島大学常三島キャンパス
    • Year and Date
      2015-09-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] 5)Axiomジェノタイピングアレイを用いた栽培イチゴの超高密度SNP連鎖地図の作成2015

    • Author(s)
      永野聡一郎、白澤健太、平川英樹、前田ふみ、石川正美、磯部祥子
    • Organizer
      園芸学会
    • Place of Presentation
      徳島大学常三島キャンパス
    • Year and Date
      2015-09-27
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Genome Sequencing Challenge to Polyploidy Crops2015

    • Author(s)
      Isobe Sachiko
    • Organizer
      Big Data and climate smart agricultue's triple challenges
    • Place of Presentation
      Jeonju, Korea
    • Year and Date
      2015-09-24
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited

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Published: 2015-04-16   Modified: 2022-08-30  

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