Pyramiding effects of high-yielding and strong-culm genes and their mechanisms for developing high-yielding rice varieties with a superior resistance to typhoon

• Project/Area Number: 15H04442
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Crop production science
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: Ookawa Taiichiro 東京農工大学, (連合)農学研究科(研究院), 教授 (80213643)
• Co-Investigator(Kenkyū-buntansha): 平沢 正 東京農工大学, (連合)農学研究科(研究院), 名誉教授 (30015119)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥15,340,000 (Direct Cost: ¥11,800,000、Indirect Cost: ¥3,540,000); Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2016: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2015: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
• Keywords: 水稲 / 倒伏抵抗性 / 強稈遺伝子 / 集積効果 / 量的形質遺伝子座 / 多収性 / イネ / 強稈 / 集積 / 多収 / 遺伝子 / 皮層繊維組織 / 収量 / ＱＴＬ / 台風 / QTL

Outline of Final Research Achievements

To evaluate the pyramiding effects of strong culm genes in rice, SCM1～SCM4 on lodging resistance,　NIL-SCM1, SCM2, SCM3 and SCM4 were developed. Then, we developed the pyramiding lines with double, triple and quadruple combinations derived from NIL-SCM1～NIL-SCM4. Quadruple pyramiding lines (NIL-SCM1+2+3+4) showed the largest culm diameter and the highest culm strength among the combinations, and increased spikelet number due to the pleiotropic effects of these genes. Pyramiding of strong culm genes resulted in increased culm thickness, culm strength and spikelet number due to their preiotropic effect. SCM1 mainly contributed to enhance their pyramiding effect due to the development of cortical fiber tissue in the surrounded region of culm. These results in this study suggest the importance of identifying the combinations of superior alleles of strong culm genes among natural variation and pyramiding these genes for improving high-yielding varieties.

Research Products

• [Journal Article] Finding the superior allele of japonica-type for increasing stem lodging resistance in indica rice varieties using chromosome segment substitution lines. (2018)
  • Author(s): Indria W. M., Yamamoto, T., Ueda, T., Samadi, A.F., Kamahora, E., Rumanti I.A., Thanh, V.C., Adachi, S., Suzuki, S., Kanekatsu, M., Hirasawa, T., Ookawa, T.
  • Journal Title: Rice Volume: 11 Issue: 1 Pages: 25-25
  • DOI: 10.1186/s12284-018-0216-3
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Precise estimation of genome regions controlling lodging resistance using a set of reciprocal chromosome segment substitution lines in rice (2016)
  • Author(s): Ookawa T, Aoba R, Yamamoto T, Ueda T, Takai T, Fukuoka S, Ando T, Adachi S, Matsuoka M, Ebitani T, Kato Y, Mulsanti IW, Kishii M, Reynolds M, Pinera F, Kotake T, Kawasaki S, Motobayashi T, Hirasawa T.
  • Journal Title: Scientific Reports Volume: 6巻 Issue: 1 Pages: 30572-30572
  • DOI: 10.1038/srep30572
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Isolation of a Novel Lodging Resistance QTL Gene Involved in Strigolactone Signaling and Its Pyramiding with a QTL Gene Involved in Another Mechanism (2015)
  • Author(s): Kenji Yano, Taiichiro Ookawa, Koichiro Aya, Yusuke Ochiai, Tadashi Hirasawa, Takeshi Ebitani, Takeshi Takarada, Masahiro Yano, Toshio Yamamoto, Shuichi Fukuoka, Jianzhong Wu, Tsuyu Ando, Reynante Lacsamana Ordonio, Ko Hirano and Makoto Matsuoka
  • Journal Title: Molecular Plant Volume: 8 Issue: 2 Pages: 303-314
  • DOI: 10.1016/j.molp.2014.10.009
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Natural variation in the flag leaf morphology of rice due to a mutation of the NARROW LEAF 1 gene in Oryza sativa L.. (2015)
  • Author(s): Taguchi-Shiobara, F., Ota, Y., Ebana,K., Ookawa, T., Yamasaki, M., Tanabata, T., Yamanouchi, U., Wu, J., Ono, N., Nonoue, Y., Nagata, K., Fukuoka, S., Hirabayashi, H., Yamamoto, T. and Yano, M.
  • Journal Title: Genetics Volume: 201 Issue: 2 Pages: 795-808
  • DOI: 10.1534/genetics.115.181040
  • NAID: 120006632721
  • Peer Reviewed / Open Access
• [Presentation] 水稲の強稈・多収関連形質の量的形質遺伝子座解析―極太稈系統LTAT-29とタカナリの組換え自殖系統群を用いて (2018)
  • Author(s): 荒川直也，野村知宏，山本敏央，上田忠正，米丸淳一，阿部陽，安達俊輔，平沢正，大川泰一郎
  • Organizer: 日本作物学会第245回講演会
• [Presentation] Development of the pyramiding lines with strong culm genes derived from crosses among the SCM near isogenic lines in Rice. (2017)
  • Author(s): Ookawa, T., Kamahora, E., Ebitani, T., Yamaguchi, Murata, K., Iyama, T., Ozaki, H., Adachi, S., Hirasawa, T. and Kanekatsu, M.
  • Organizer: 第9回アジア作物学会議
  • Int'l Joint Research
• [Presentation] Estimation of the Quantitative trait loci Associated with Breaking and Bending Types Lodging Resistance in Rice Using Chromosome Segment Substitution Lines Derived from a Cross between Takanari and Koshihikari (2017)
  • Author(s): Indria, W.M., Yamamoto, T., Ueda, T., Samadi, A.F., Adachi, S., Hirasawa, T. and Ookawa, T.
  • Organizer: 第9回アジア作物学会議
  • Int'l Joint Research
• [Presentation] Identification of the Quantitative Trait Loci for Breaking and Bending Types Lodging Resistance in Rice, Using Recombinant Inbred Lines Derived from Koshihikari and a Strong Culm Variety, Leaf Star (2017)
  • Author(s): Samadi,A.F., Yamamoto, T., Ueda, T., Adachi, S., Hirasawa, T. and Ookawa, T.
  • Organizer: 第9回アジア作物学会議
  • Int'l Joint Research
• [Presentation] 異なる水稲品種に由来する強稈遺伝子の組合せが強稈関連形質の集積効果に及ぼす影響 (2017)
  • Author(s): 釜洞 瑛里, 蛯谷 武志, 伊山 幸秀, 松岡 信, 矢野 憲司, 安達 俊輔, 平沢 正, 大川 泰一郎
  • Organizer: 日本作物学会第243回講演会
• [Presentation] 水稲の強稈および収量関連形質に関わる量的形質遺伝子座のコシヒカリへの集積効果 (2016)
  • Author(s): 吉村 大, 蛯谷 武志, 伊山 幸秀, 平沢 正, 安達 俊輔, 大川 泰一郎
  • Organizer: 日本作物学会第241回講演会
  • Place of Presentation: 日本大学（神奈川県藤沢市）
  • Year and Date: 2016-03-27
• [Presentation] 水稲の倒伏抵抗性に関与する強稈関連形質の量的形質遺伝子座の特定－リーフスターとコシヒカリの組換え自殖系統を用いて－ (2016)
  • Author(s): Ahmad Fahim Samadi, 上田 忠正, 山本 敏央, 安達 俊輔, 平沢 正, 大川 泰一郎
  • Organizer: 日本作物学会第241回講演会
  • Place of Presentation: 日本大学（神奈川県藤沢市）
  • Year and Date: 2016-03-27
• [Presentation] タカナリを遺伝背景とするコシヒカリの染色体断片置換系統における挫折型およびたわみ型倒伏抵抗性に関わる量的形質遺伝子座の特定 (2016)
  • Author(s): Indria Wahyu Mulsanti, 山本 敏央, 上田 忠正, 田原 佳奈子, 安達 俊輔, 平沢 正, 大川 泰一郎
  • Organizer: 日本作物学会第241回講演会
  • Place of Presentation: 日本大学（神奈川県藤沢市）
  • Year and Date: 2016-03-27
• [Presentation] 水稲の強稈質に関わる皮層繊維組織を厚くする量的形質遺伝子座の特定―タカナリを遺伝背景とするコシヒカリ第2, 9染色体の組換え固定系統を用いて― (2016)
  • Author(s): 神山 遼, 山本 敏央, 上田 忠正, 福岡 修一, 安藤 露, 平沢 正, 安達 俊輔, 大川 泰一郎
  • Organizer: 日本作物学会第241回講演会
  • Place of Presentation: 日本大学（神奈川県藤沢市）
  • Year and Date: 2016-03-27
• [Remarks] 東京農工大学農学部生物生産学科作物学教育研究分野
  • URL: http://web.tuat.ac.jp/~crop/