| Budget Amount *help |
¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000)
Fiscal Year 2017: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
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| Outline of Final Research Achievements |
Soybean is sensitive to flooding stress, which markedly reduces its growth. To identify the mechanism of flooding tolerance at initial stage in early-stage soybean, omics techniques and simulation analysis were used. Flooding tolerant mutant line and abscisic acid (ABA)-treated soybean, which exhibited flooding tolerant phenotype, were used as materials. Soybean roots were collected for proteomic as well as metabolomic and transcriptomic analyses. Commonly changed metabolites, proteins, and genes between mutant and ABA-treated soybeans were considered as flooding-tolerance related candidate factors. Finally, omics results were integrated to analyze the flooding tolerant mechanism in soybean at initial stage. Integrated omics suggests that fructose might be the critical metabolite through regulation of hexokinase/phosphofructokinase and the regulation of energy metabolism might be crucial step to confer initial-flooding tolerance in early-stage soybean.
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