Molecular breeding of floricultural crops for greening of petals and flower life extension and their mechanisms
| Project/Area Number |
15H04449
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Horticultural science
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2017: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2016: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
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| Keywords | 花弁緑色化 / 花持ち性向上 / 花形改変 / 分子育種 / オミクス / 花弁特的プロモーター |
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| Outline of Final Research Achievements |
Petal specific InMYB1 promoter from morning glory functions recognizing petal identity at cell level, but not whole2 position in shoot apical meristem. In the upstream region of InMYB1, the 332-121 b upstream region contains cis-element, which defines petal identity. We produced the vector harboring 3 repeats of the 332-121 b upstream region of InMYB1 for molecular breeding of floricultural crops. GPP gene from rice can induce greening, morphological change and life extension in the petals of Arabidopsis, morning glory and petunia. These changes caused by addition of leaf identity to petals.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究において，アサガオ由来花弁特的InMYB1プロモーターが，花弁のidentityを細胞レベルで認識して作動すること，InMYB1上流332-121 bに花弁特異性を規定する領域が存在することを明らかにしたことは，植物生理学的における学術的意義が大きい．また，このInMYB1プロモーターベースとし，花弁特異的な遺伝子発現を誘導する形質転換ベクターを開発し，アサガオやペチュニアの花を緑色化すると共に，花弁の老化を遅延できることを示したことは，花きの分子育種の発展に大きく貢献するものであり，その社会的意義は大きい．
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Report
(4 results)
Research Products
(23 results)
