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Genetic engineering of betalain pigments beyond plant species

Research Project

Project/Area Number 15H04453
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Horticultural science
Research InstitutionIwate Biotechnology Research Center

Principal Investigator

Nishihara Masahiro  公益財団法人岩手生物工学研究センター, 園芸資源研究部, 研究部長 (20390883)

Co-Investigator(Kenkyū-buntansha) 高橋 秀行  公益財団法人岩手生物工学研究センター, 園芸資源研究部, 主任研究員 (00455247)
佐々木 伸大  公益財団法人岩手生物工学研究センター, 園芸資源研究部, 主任研究員 (80422088)
Co-Investigator(Renkei-kenkyūsha) SASAKI Nobuhiro  東洋大学, 食環境科学部食環境科学科, 准教授 (80422088)
Research Collaborator Higuchi Atsumi  公益財団法人 岩手生物工学研究センター, 園芸資源研究部, 研究助手
FUJITA Kohei  公益財団法人 岩手生物工学研究センター, 園芸資源研究部, 研究助手
WATANABE Aiko  公益財団法人 岩手生物工学研究センター, 園芸資源研究部, 研究助手
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Keywords植物色素 / 遺伝子組換え / 花色 / ゲノム編集 / べタレイン / 代謝工学 / ベタレイン
Outline of Final Research Achievements

Betalains are plant pigments that specifically accumulate in plant species belonging to the Caryophyllales and they show from yellow to red-violet colors. There is a mutually exclusive nature between betalain and anthocyanin pigments in the plant kingdom. In this study, we attempted to produce betalain pigments, yellow betaxanthins and red-violet betacyanins, in non-betalain-synthesizing plant species by genetic transformation of betalain biosynthetic genes.
As the results, successful production of betalain pigments has been achieved in leaves, flowers, fruits and tubers in various plant species such as tobacco, torenia, tomato and potato. The established methods are useful for production of novel flower-colored plants lacking red and yellow colors. They are also useful for production of food crops benefic for the human health and studies of the biological roles of betalain pigments in higher plants in the future.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (10 results)

All 2018 2017 2016 2015

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (8 results) (of which Int'l Joint Research: 1 results,  Invited: 3 results) Book (1 results)

  • [Journal Article] Development of basic technologies for improvement of breeding and cultivation of Japanese gentian2018

    • Author(s)
      Masahiro Nishihara, Keisuke Tasaki, Nobuhiro Sasaki, Hideyuki Takahashi
    • Journal Title

      Breeding Science

      Volume: 68 Issue: 1 Pages: 14-24

    • DOI

      10.1270/jsbbs.17074

    • NAID

      130006685377

    • ISSN
      1344-7610, 1347-3735
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] CRISPR/Cas9-mediated targeted mutagenesis of flavanone 3-hydroxylase gene in tobacco, torenia and gentian plants.2018

    • Author(s)
      Tasaki, K., Watanabe, A., Higuchi, A., Kurokawa, Y., Washiashi, R., Takahashi, H., Nishihara, N.
    • Organizer
      日本植物生理学会第59回年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 植物種を超えたベタレイン色素のエンジニアリング2017

    • Author(s)
      西原昌宏
    • Organizer
      アグリ・バイオ公開シンポジウム Division of Agri-biotechnology、東京理科大学研究推進機構総合研究院 アグリ・バイオ工学研究部門
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] ゲノム編集技術によるリンドウ花色変異体の作出と解析2017

    • Author(s)
      田崎啓介、樋口敦美、渡辺藍子、黒川良美、鷲足理恵、佐々木伸大、西原昌宏
    • Organizer
      園芸学会平成29年度秋季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 植物のベタレイン合成のメカニズムとその役割2016

    • Author(s)
      佐々木伸大
    • Organizer
      園芸学会平成28年度秋季大会 公開シンポジウム 「園芸植物がもつ色素の機能と可能性」
    • Place of Presentation
      名城大学天白キャンパス
    • Year and Date
      2016-09-10
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] タバコ及びトレニアにおけるベタレイン色素のエンジニアリング2016

    • Author(s)
      西原昌宏、山田恵理、樋口敦美、藤田晃平、黒川良美、中塚貴司、 宮原平、小関良宏、高橋秀行、佐々木伸大
    • Organizer
      日本植物細胞分子生物学会第34回大会
    • Place of Presentation
      信州大学繊維学部
    • Year and Date
      2016-09-01
    • Related Report
      2016 Annual Research Report
  • [Presentation] 西原昌宏、田崎啓介、樋口敦美,藤田晃平、黒川良美、高橋秀行、佐々木伸大2016

    • Author(s)
      CRISPR/Cas9システムによるタバコ及びトレニアの花色改変
    • Organizer
      日本植物生理学会第57回年会
    • Place of Presentation
      岩手大学 上田キャンパス
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] Application of CRISPR/Cas9 System for Modification of Flower Colors in Tobacco and Torenia Plants.2016

    • Author(s)
      西原昌宏、樋口敦美,藤田晃平、田崎啓介、黒川良美、高橋秀行、佐々木伸大
    • Organizer
      Plant and Animal Genome XXIV Conference
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2016-01-14
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] リンドウの花色生合成機構の解明と新規花色創出へ向けての取組み2015

    • Author(s)
      西原昌宏
    • Organizer
      第52回植物化学シンポジウム オミクス解析を用いた有用な遺伝子・代謝産物の探索と活用
    • Place of Presentation
      東京大学薬学部講堂
    • Year and Date
      2015-11-13
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Book] 植物のたくらみ ー香りと色の植物学2018

    • Author(s)
      有村源一郎、西原昌宏
    • Total Pages
      159
    • Publisher
      ベレ出版
    • ISBN
      9784860645441
    • Related Report
      2017 Annual Research Report

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Published: 2015-04-16   Modified: 2019-03-29  

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