| Project/Area Number |
15H04454
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Horticultural science
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| Research Institution | Iwate Biotechnology Research Center |
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Principal Investigator |
Takahashi Hideyuki 公益財団法人岩手生物工学研究センター, 園芸資源研究部, 主任研究員 (00455247)
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| Co-Investigator(Kenkyū-buntansha) |
西原 昌宏 公益財団法人岩手生物工学研究センター, 園芸資源研究部, 研究部長 (20390883)
金野 尚武 宇都宮大学, 農学部, 准教授 (60549880)
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| Research Collaborator |
FUJITA KOUHEI 公益財団法人 岩手生物工学研究センター, 園芸資源研究部, 研究助手
YOSHIDA CHIHARU 公益財団法人 岩手生物工学研究センター, 園芸資源研究部, 研究助手
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2017: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2016: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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| Keywords | 花卉 / 開花 / 休眠 / 成長相転換 / リンドウ |
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| Outline of Final Research Achievements |
Gentian (Gentiana triflora) is an herbaceous perennial that possesses several growth phases, including germination, growth, and dormancy. This study aimed to reveal the relationship between gentio-oligosaccharide metabolism and growth phase transitions. Metabolome and RNA-seq analyses revealed that several metabolites and genes were fluctuated during each phase transition, and some genes related to phase transition were induced by gentio-oligosaccharide application, implying that the oligosaccharides related to phase transition in gentian. Furthermore, we identified gentio-oligosaccharide degradative enzyme, GtGen3A, which is involved in controlling gentiobiose concentration. Because genetic modification of genes found in this study, including Gtgen3A, seemed to affect developmental processes, more detailed analysis will reveal the regulatory mechanisms of phase transition mediated by gentio-oligosaccharides.
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