Analysis of quorum sensing-meditated regulation of T3SS gene expression
| Project/Area Number |
15H04458
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant protection science
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| Research Institution | Okayama University |
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Principal Investigator |
ICHINOSE Yuki 岡山大学, 環境生命科学研究科, 教授 (50213004)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2017: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
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| Keywords | 菌体密度感知機構 / アシルホモセリンラクトン / PsyR / 転写制御 / タイプIII分泌システム / Hrp / Gac2成分制御系 / rsmX / Gac２成分制御系 / 病原性 |
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| Outline of Final Research Achievements |
The mechanisms of acyl-homoserinelactone (AHL)-mediated quorum sensing (QS) in Pseudomonas syringae was investigated. Recombinant PsyR of P. syringae pv. tabaci 6605 (Pta6605) bound to the promoter of AHL synthase gene, psyI without AHL, and activated its transcription with AHL. PsyR also bound to the imperfect inverted repeat located in the promoter of hrpL, a gene for transcriptional activator of hrp type III secretion system genes. Pta6605 and P. syringae pv. tomato DC3000 highly expressed noncoding small regulatory RNAs, rsmZ and rsmY at high cell density conditions. Because PtoDC3000 doesn’t produced AHL, importance of Gac/Rsm signaling pathway in QS was suggested.
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Report
(4 results)
Research Products
(24 results)
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[Journal Article] MexEF-OprN multidrug efflux pump transporter negatively controls N-acyl-homoserine lactone accumulation in Pseudomonas syringae pv. tabaci 66052018
Author(s)
Sawada, T., Eguchi, M., Asaki, S., Kashiwagi, R., Shimomura, K., Taguchi, F., Matsui, H., Yamamoto, M., Noutoshi, Y., Toyoda K., and Ichinose, Y.
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Journal Title
Molecular Genetics and Genomics
Volume: 未定
Issue: 4
Pages: 907-917
DOI
NAID
Related Report
Peer Reviewed / Open Access
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