| Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2017: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
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| Outline of Final Research Achievements |
The mechanisms of acyl-homoserinelactone (AHL)-mediated quorum sensing (QS) in Pseudomonas syringae was investigated. Recombinant PsyR of P. syringae pv. tabaci 6605 (Pta6605) bound to the promoter of AHL synthase gene, psyI without AHL, and activated its transcription with AHL. PsyR also bound to the imperfect inverted repeat located in the promoter of hrpL, a gene for transcriptional activator of hrp type III secretion system genes. Pta6605 and P. syringae pv. tomato DC3000 highly expressed noncoding small regulatory RNAs, rsmZ and rsmY at high cell density conditions. Because PtoDC3000 doesn’t produced AHL, importance of Gac/Rsm signaling pathway in QS was suggested.
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