Elucidation of the mechanism of serotonin accumulation in rice root; A suppression of serotonin concentration during a primary stage.

• Project/Area Number: 15H04462
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant protection science
• Research Institution: Kochi University
• Principal Investigator: Tebayashi Shinichi 高知大学, 教育研究部自然科学系農学部門, 准教授 (70325405)
• Co-Investigator(Kenkyū-buntansha): 間世田 英明 国立研究開発法人産業技術総合研究所, 生命工学領域, 上級主任研究員 (10372343) ; 及川 彰 山形大学, 農学部, 准教授 (50442934) ; 石原 亨 鳥取大学, 農学部, 教授 (80281103)
• Research Collaborator: Oonishi Shintaro ; Ueda Shinji ; Yamashita Saki ; Sumida Hayato
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000); Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2016: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2015: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
• Keywords: rice / aphid / イネ / アミノ酸 / アブラムシ / セロトニン / 蓄積 / 抑制 / 防御機構 / メタボローム / オカボノアカアブラムシ / 褐変

Outline of Final Research Achievements

The rice root aphid, Rhopalosiphum rufiabdominalis, can suppress a serotonin concentration during a primary stage, although a high amount serotonin was normally induced by a pathogen attacking. The serotonin production by tryptophan decarboxylase could be regulated by both of transcription factor WRKY26 and WRKY14. When an aphid attacked a rice root, isopentenyladenine provided by an aphid would induce WRKY26 but suppress WRKY14 on the root attacked by an aphid, resultingly serotonin would be kept at a low concentration. On the other hand, a coexpression of WRKY26 and WRKY14 would lead a high concentration of serotonin at a middle phase.

Academic Significance and Societal Importance of the Research Achievements

昆虫-植物相互作用においてアブラムシがイネの防御反応を意図的に抑制することで抵抗性を弱めるばかりか、さらにこの抵抗性物質を逆手にとり自身の増殖に積極的に利用していることを見出し、その制御機構を解明することに成功した。このように食植者が寄主植物の栄養状態を改善するメカニズムは、昆虫-植物の寄主選択に新たな概念となり意義深い。また現在まで昆虫に散発的に存在するサイトカイニン類の機能は不明であったが、今回の成果はその答えの一つとなり、昆虫由来サイトカイニンの機能解明の端緒となり、これが普遍的な現象であれば昆虫-植物の相互作用のメカニズムに新たな常識を確立することが出来る。

Research Products

• [Journal Article] Accumulation of 9- and 13-KODEs in response to jasmonic acid treatment and pathogenic infection in rice, (2018)
  • Author(s): Nishiguchi, S, Murata, K, Ube, N, Ueno, K, Tebayashi, S, Teraishi, M, Okumoto, Y, Mori, N, Ishihara,
  • Journal Title: Journal of Pesticide Science Volume: 81 Pages: 191-197
  • NAID: 130007437390
  • Peer Reviewed
• [Journal Article] Induced accumulation of tyramine, serotonin, and related amines in response to Bipolaris sorokiniana infection in barley. (2017)
  • Author(s): Atsushi Ishihara, Rie Kumeda, Noriko Hayashi, Yukari Yagi, Nanase Sakaguchi, Yu Kokubo, Naoki Ube, Shin-ichi Tebayashi, Kotomi Ueno
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 印刷中 Issue: 6 Pages: 1090-1098
  • DOI: 10.1080/09168451.2017.1290520
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Distribution of the tryptophan-pathway-derived defensive secondary metabolites gramine and benzoxazinones in Poaceae. (2017)
  • Author(s): Kokubo Y, Nishizaka M, Ube N, Yabuta Y Tebayashi SI, Ueno K, Taketa S, Ishihara A
  • Journal Title: Biosci Biotechnol Biochem. Volume: 81 Issue: 3 Pages: 431-440
  • DOI: 10.1080/09168451.2016.1256758
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Studies on the Probing Stimulants for the White-backed Planthopper, Sogatella furcifera (Homoptera: Delphacidae) in Rice Plant (Oryza sativa L.) (2016)
  • Author(s): Zhan ZH, Matsuo A, Oku Y, Tebayashi SI, Kim CS.
  • Journal Title: Biosci Biotechnol Biochem Volume: 80 Issue: 12 Pages: 2285-2290
  • DOI: 10.1080/09168451.2016.1220820
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Induced resistance on the root of rice plant by plant hormones against root aphid. (2018)
  • Author(s): Shinichi Tebayashi*1, Hayato Sumita1, Akira OIKAWA2, Hideaki MASEDA3, Atsushi ISHIHARA4
  • Organizer: ISCE 34th Annual Meeting, Budapest, Hungary
  • Int'l Joint Research
• [Presentation] オカボノアカアブラムシのイネ根への寄生によるアミノ酸の蓄積 (2017)
  • Author(s): 手林 慎一，佐野千聡、大西 信太郎，及川 彰， 佐々木 亮介， 斉藤 和季， 上手 麻希， 間世田 英明， 石原 亨
  • Organizer: 日本農芸化学会2016年度大会
  • Place of Presentation: 京都
  • Year and Date: 2017-03-17
• [Presentation] オカボノアカアブラムシのイネ根への寄生による褐変機構の解明 (2016)
  • Author(s): 山下 紗季，手林 慎一， 上田 真二，及川 彰， 佐々木 亮介， 斉藤 和季， 上手 麻希， 間世田 英明， 石原 亨
  • Organizer: 植物化学調節学会第51回大会
  • Place of Presentation: 高知
  • Year and Date: 2016-10-28
• [Presentation] オカボノアカアブラムシのイネ根への寄生による褐変機構の解明 (2016)
  • Author(s): 手林 慎一1， 上田 真二1， 森 梓紗1， 及川 彰2,3， 佐々木 亮介3， 斉藤 和季3,4， 上手 麻希5， 間世田 英明5， 石原 亨
  • Organizer: 本農芸化学会2016年度大会
  • Place of Presentation: 札幌
  • Year and Date: 2016-03-27
• [Presentation] BROWNING MECHANISM ON THE ROOTS OF RICE PLANT INFESTED BY RICE ROOT APHID, RHOPALOSIPHUM RUFIABDOMINALIS, AND EFFECTS OF SALICYLIC ACID. (2015)
  • Author(s): Azusa Mori1, Shinichi Tebayashi1, Shinji UEDA1, Akira OIKAWA2,5, Ryosuke, SASAKI5, Maki UWATE3, Hideaki MASEDA3, Kazuki SAITO5,6, Atsushi ISHIHARA4;
  • Organizer: he International Chemical Congress of Pacific Basin Societies 2015
  • Place of Presentation: Honolulu
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] BROWNING MECHANISM ON THE ROOTS OF RICE PLANT ATTACKED BY RICE ROOT APHID, RHOPALOSIPHUM RUFIABDOMINALIS. (2015)
  • Author(s): Shinichi Tebayashi1, Chisato SANO, Shinji UEDA, Akira OIKAWA, Ryosuke, SASAKI, Maki UWATE, Hideaki MASEDA, Kazuki SAITO, Atsushi ISHIHARA
  • Organizer: International Chemical Ecology Conference 2015
  • Place of Presentation: Stockholm
  • Year and Date: 2015-06-29
  • Int'l Joint Research
• [Remarks] 高知大学農林海洋科学部化学生態学研究室
  • URL: http://www.cc.kochi-u.ac.jp/~tebayasi/