Elucidation of molecular mechanisms underlying the growth suppression of rice plants at elevated CO2 concentrations and development of techniques for releasing the suppression

• Project/Area Number: 15H04470
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: Tohoku University
• Principal Investigator: TOKUTOMI Mitsue 東北大学, 農学研究科, 教授 (70181980)
• Research Collaborator: KIM Yonghyun 東北大学, 大学院農学研究科, 研究支援者 ; INOUE Masae 名古屋工業大学, 大学院工学研究科, 博士研究員 (60459732)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000); Fiscal Year 2017: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2015: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
• Keywords: 高CO2環境 / 葉の発達段階 / C/Nバランス / イネ

Outline of Final Research Achievements

The elevated CO2 concentrations in the atmosphere are transmitted from leaves exposed to elevated CO2 to other organs via a long-distance signal. To identify the signal and the signal-transduction system, transcriptomes of the signal sender (fully developed leaves; P6 stage when P0 represents the stage of leaf primordium initiation) and the signal recipient (developing leaves at the P4 stage) were compared. Results suggest some candidate molecules for the signal, namely, a low-molecular-weight protein and some miRNA species. From the effects of sugar feeding to fully developed leaves, it was found that sugars synthesized in leaves exposed to elevated CO2 could not be the signal but acted as carbon nutrients required for responses to elevated CO2.

Research Products

• [Journal Article] Characterization and expression analyses of two plastidic enolase genes in rice (2015)
  • Author(s): Fukayama, H., Masumoto, C., Taniguchi, Y., Baba-Kasai, A., Katoha, Y., Ohkawa, H. and Miyao, M.
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: 79 Issue: 3 Pages: 402-409
  • DOI: 10.1080/09168451.2014.980219
  • Peer Reviewed
• [Journal Article] Comparison of plant-type phosphoenolpyruvate carboxylases from rice: Identification of two plant-specific regulatory regions of the allosteric enzyme (2015)
  • Author(s): Muramatsu, M., Suzuki, R., Yamazaki, T. and Miyao, M.
  • Journal Title: Plant Cell Physiol. 56: 468-480 Volume: 56 Issue: 3 Pages: 468-480
  • DOI: 10.1093/pcp/pcu189
  • Peer Reviewed
• [Presentation] イネにおけるトレハロース代謝関連遺伝子の発現に対する高CO2の効果：成熟葉と未熟葉の比較 (2018)
  • Author(s): 金容賢，今野雅恵，徳富（宮尾）光恵
  • Organizer: 第59回日本植物生理学会年会
• [Presentation] Selection of rice cultivars showing different nitrogen responses from NIAS core collections and their transcriptomic comparison (2018)
  • Author(s): Kim, Y., Yanagisawa, S., Miyao-Tokutomi, M.
  • Organizer: 第59回日本植物生理学会年会
• [Presentation] 水田で栽培したイネ品種の窒素栄養状態と葉温の相関 (2017)
  • Author(s): 菅野圭一，金容賢，柳澤修一，宮尾光恵
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学（鹿児島県鹿児島市）
  • Year and Date: 2017-03-16
• [Presentation] イネ品種間で見られる窒素飢餓応答とリン酸飢餓応答の多様性のリン酸取り込みを指標とした評価 (2017)
  • Author(s): 植田佳明，宮尾（徳富）光恵，柳澤修一
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学（鹿児島県鹿児島市）
  • Year and Date: 2017-03-16
• [Presentation] サイトゾル型グルタミン合成酵素1;2 を欠損したイネ変異体の分げつの減少はアスパラギンよりもグルタミンの利用可能量の減少に起因する (2017)
  • Author(s): 大橋美和，石山敬貴，小島創一，小西範幸，宮尾光恵，山谷知行，早川俊彦
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学（鹿児島県鹿児島市）
  • Year and Date: 2017-03-16
• [Presentation] 栄養素の取り込みを指標としたイネの窒素・リン飢餓応答性の品種間差の解析 (2017)
  • Author(s): 植田佳明，宮尾（徳富）光恵，柳澤修一
  • Organizer: 日本土壌肥料学会2017年度仙台大会
• [Presentation] イネ根におけるアンモニア同化：土耕と水耕の比較 (2016)
  • Author(s): 宮澤真一，今野雅恵，村松昌幸，宮尾光恵
  • Organizer: 第2回植物の栄養研究会
  • Place of Presentation: 名古屋大学（愛知県名古屋市）
  • Year and Date: 2016-09-02
  • Invited
• [Presentation] Chloroplastic glutamine synthetase (GS2), a crucial enzyme for photorespiration, is absent in needle-leaved tree species (2016)
  • Author(s): Miyazawa, S.-I., Miyao-Tokutomi, M. and Futamura, N.
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学（岩手県盛岡市）
  • Year and Date: 2016-03-18
• [Presentation] Survey of signaling molecules that transduce elevated CO2 environments in rice plants: effects of soluble sugar addition on leaf blade size. (2016)
  • Author(s): Kim, Y., Konno, M. and Miyao-Tokutomi, M.
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学（岩手県盛岡市）
  • Year and Date: 2016-03-18