| Budget Amount *help |
¥15,340,000 (Direct Cost: ¥11,800,000、Indirect Cost: ¥3,540,000)
Fiscal Year 2017: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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| Outline of Final Research Achievements |
To investigate a PCB degrader Acidovorax sp. KKS102, a method named qTnSeq was developed. We revealed strong 3' tailing activity of a reverse transcriptase, and a series of tailing enhancers. We also optimized an unique reaction named CIS reaction. In the CIS reaction, a single strand DNA with 3' end sequence complementary to the tail is incorporated with concomitant DNA polymerization starting from the 3' tail. We designed and created transposon cassette for qTnSeq, and constructed a Tn mutant library consisting of about 60,000 clones, and we demonstrated the usefulness of the qTnSeq method. In addition, we investigated Acidovorax sp. KKS102 with respect to its catabolite control mechanism and a mobile genetic element that carries PCB-degrading genes.
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